Project description:MPTP treated Macaca fascicularis prefrontal cortices compared to saline treated and untreated controls Experiment Overall Design: Macaques injected daily with Experiment Overall Design: MPTP hydrochloride (0.2 mg/kg, or with saline. Each group consisted of
Project description:To characterize both short- and long-term (7 days and 28 days) gene expression profiling differences between control and MPTP-treated retina, we determined levels of gene expression using microarray analysis and real-time PCR. We found 60 genes relatively regulated in retinas treated with MPTP for 7 days (26 up-regulated and 34 down-regulated), whereas 54 genes were regulated in retinas treated with MPTP for 28 days (18 up-regulated and 36 down-regulated) when compared with the non-treated retina in each control groups. Total 26 annotated differently expressed genes were chosen for further validation by quantitative real-time PCR, and 4 genes in the 7-day treatment group (Clec2e; Dio2; Hmcn1; Rlbp1) and 3 genes in the 28-days treatment group (Pnmt; Tmem121; Ssxb3) were confirmed.
Project description:To characterize more broadly the effect of attenuated TLR4 signaling responses in sooty mangabeys, we performed a comparative RNA-seq profiling of LPS-treated primary monocytes from rhesus macaques and sooty mangabeys. Production of TNF- and IL6 mRNA was significantly lower in sooty mangabeys. Moreover, we observed that induction of NF-κB -regulated inflammatory genes was broadly and significantly reduced in sooty mangabeys, for TNF-a and IL-6 signaling pathways, compared to rhesus macaques. Overall, these data indicate that LPS stimulation of SM blood cells results in a significantly blunted production of pro-inflammatory cytokines as compared to rhesus macaque macrophages.
Project description:To characterize both short- and long-term (7 days and 28 days) gene expression profiling differences between control and MPTP-treated retina, we determined levels of gene expression using microarray analysis and real-time PCR. We found 60 genes relatively regulated in retinas treated with MPTP for 7 days (26 up-regulated and 34 down-regulated), whereas 54 genes were regulated in retinas treated with MPTP for 28 days (18 up-regulated and 36 down-regulated) when compared with the non-treated retina in each control groups. Total 26 annotated differently expressed genes were chosen for further validation by quantitative real-time PCR, and 4 genes in the 7-day treatment group (Clec2e; Dio2; Hmcn1; Rlbp1) and 3 genes in the 28-days treatment group (Pnmt; Tmem121; Ssxb3) were confirmed. Male C57BL/6 mice were randomly divided into four treatment groups (n= 6 in each group): the 7 days and 28 days saline-injection (control) groups (C-7 and C-28, respectively), and 7 days and 28 days MPTP-injection groups (M-7 and M-28, respectively). 4 retinas for 1 samples (1 gene chip), (4 experimental group × 2 samples of each experimental group = total 8 samples).
Project description:Herein, we used rhesus macaques (RMs) infected with SIVmac251 to analyze the transcriptomic profiles of the liver and lungs of untreated and early antiretroviral therapy (ART)-treated RMs, compared to healthy uninfected RMs.