Gossypium hirsutum
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ABSTRACT:
PROVIDER: PRJNA95895 | ENA |
REPOSITORIES: ENA
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Project description:This study was initiated with the objective of identifying the anther/tapetum specific promoters from cotton floral buds. Cotton is an important commercial crop. Hybrid cotton varieties are developed to obtain improved yield and fiber quality. Most of the hybrid seed production in cotton is carried out by hand emasculation, which requires large amount of manpower, resulting in high cost of hybrid seed. We are developing barnase-barstar based male sterility system, which would be a better alternative for hybrid development. The tapetum specific promoters are main requirement for such a system. The study was thus carried out to identify genes expressed in the anthers.
2013-10-31 | GSE51779 | GEO
Project description:This study was initiated with the objective of identifying the anther/tapetum specific promoters from cotton floral buds. Cotton is an important commercial crop. Hybrid cotton varieties are developed to obtain improved yield and fiber quality. Most of the hybrid seed production in cotton is carried out by hand emasculation, which requires large amount of manpower, resulting in high cost of hybrid seed. We are developing barnase-barstar based male sterility system, which would be a better alternative for hybrid development. The tapetum specific promoters are main requirement for such a system. The study was thus carried out to identify genes expressed in the anthers. Cotton bud sizes were correlated with tapetum development. RNA was isolated from following tissues: • Anther tissues from buds at pre-meiotic stage of development (Tapetum absent) • Buds without anther tissues at pre-meiotic stage of development • Anther tissues from buds during meiosis (Tapetum present) • Buds without anther tissues during meiosis • Anther tissues from buds at post-meiotic stage of development (Tapetum degenerated) • Buds without anther tissues at post-meiotic stage of development • Leaf tissues • Seedling 5 days after germination Biotin labeled cRNA was hybridized on Affymertix cotton Genechip Genome array following Affymetrix protocols. Three biological replicates were maintained.
2013-10-31 | E-GEOD-51779 | biostudies-arrayexpress
Project description:We compared different days post-anthesis (5DPA, 10DPA, 15DPA and 25DPA) differentially expressed genes (DEGs) in fiber development between G. hirsutum and G. barbadense. In addition, we analysis the differentially expressed genes (DEGs) function using the database for annotation, visualization and integrated discovery (DAVID). Overall, gene expression pattern have significantly difference between G. hirsutum and G. barbadense. In this study, G. barbadense DEGS in different two DPA are significantly more than G. hirsutum. In addation, there are 18937 DEGs were identified in fruit development and postembryonic development pathways and only upregulated in G. barbadense only. Taken together, these findings suggest that there are considerable differences of gene expression between G. hirsutum and G. barbadense in cotton fiber development different stages.
2021-10-23 | GSE95146 | GEO
Project description:Transcriptome analysis in cotton under drought stress. To study the molecular response of drought stress in cotton under field condition global gene expression analysis was carried out in leaf tissue. Gossypium hirsutum cv. Bikaneri Nerma was used for the gene expression analysis. Cotton plants were subjected to drought stress at peak flowering stage. Leaf samples were collected when the soil moisture content was 19.5% which is 50% of the normal control plots. Gene expression profiles in drought induced and their respective control samples were analyzed using Affymertix cotton Genechip Genome arrays to study the global changes in the expression of genome.
2012-06-04 | GSE29566 | GEO