Project description:Genomewide mapping of Drosophila Lmd protein binding during embryonic development. Two consecutive timepoints (6-8 and 8-10 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Lmd protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
Project description:Genomewide mapping of Drosophila Tinman protein binding during embryonic development. Three consecutive timepoints (2-4, 4-6, 6-8 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Tinman protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
Project description:We sequenced mRNA from 24 single D. melanogaster embryos (12 male and 12 female) taken from 8 early embryonic timepoints to generate the first sex specific timecourse of gene expression in early Drosophila development
Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster. Heat shock factor IP DNA from non-shock (room temperature) Kc 167 cells compared to whole cell extract on Agilent 2x244k tiling arrays.
Project description:Genomewide mapping of Drosophila Mef2 protein binding during embryonic development. Five consecutive timepoints (2-4, 4-6, 6-8, 8-10, 10-12 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Mef2 protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster. Heat shock factor IP DNA or Mock IP DNA from heat shocked Kc 167 cells compared to whole cell extract on Agilent 2x244k tiling arrays.
Project description:Genomewide mapping of Drosophila Twist protein binding during embryonic development. Two consecutive timepoints (2-4 and 4-6 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Twist protein. Additionally, preimmune-serum was used as a control. The enriched DNA was hybridized to custom designed tiling arrays optimized for assaying all E-box motifs outside repetitive or coding regions of the Drosophila melanogaster genome (average gap size = 290 bps).
Project description:Genomewide mapping of D. melanogaster Bagpipe protein binding during embryonic development. One time point (6-8 hrs after egg-laying) were assayed in four independent repeats. Two different antibodies were used to precipitate the Bagpipe protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
