Project description:DJ-1 expression protects H157 cells from cell death, using siRNA oligomers we knocked down DJ-1 and evaulated gene expression using Affymetrix U133A GeneChip arrays. Keywords: siRNA comparison
Project description:Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma (Hirao et al., JPR 2014, PMID: 25244057). Re-searched original MS2 data using the latest database (UniprotKB) in 2017.
Project description:DJ-1 expression protects H157 cells from cell death, using siRNA oligomers we knocked down DJ-1 and evaulated gene expression using Affymetrix U133A GeneChip arrays. Experiment Overall Design: H157 cells were transfected every 24 hours with siRNA oligomers for 3 consecutive days, lysed 96 hours after the first transfection, and total RNA extracted. cRNA was generated and used to probe Affymetrix U133A GeneChip microarrays.
Project description:This project aims at the detection of specific patterns of miRNAs in peripheral blood samples of lung cancer patients. As controls, blood of donors without known affection have been tested. Using the miRNA patterns we hope to detect a diagnostic pattern for the non-invasive diagnosis of non-small cell lung carcinoma.
