Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA96175 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Using a transgenic line expressing HA-tagged ATAF1 uncovered >400 ChIP-seq peaks in ATAF1-HA plants compared to Col-0 wild-type plants. Only a small sub-set of the candidate peaks could be verified using ChIP-qpcr or EMSA. Among the verified peaks we uncovered the key ABA biosynthetic gene NCED3 as a target of ATAF1
2014-01-01 | GSE43637 | GEO
Project description:Using a transgenic line expressing HA-tagged ATAF1 uncovered >400 ChIP-seq peaks in ATAF1-HA plants compared to Col-0 wild-type plants. Only a small sub-set of the candidate peaks could be verified using ChIP-qpcr or EMSA. Among the verified peaks we uncovered the key ABA biosynthetic gene NCED3 as a target of ATAF1 ChIP was performed using anti-HA antibodies on wild-type Col-0 plants and plants expressing HA-tagged ATAF1
2014-01-01 | E-GEOD-43637 | biostudies-arrayexpress
Project description:Transcriptional profiling of Arabidopsis thaliana 12-days old seedlings comparing Col-0 wild type with transgenic plants with altered expression of dual-targetting plastid/mitochondrial organellar RNA-polymerase RPOTmp. Transgenic plants used for experiment were: overexpressor plants obtained by transformation of Col-0 WT plants with genetic constructs created in [Tarasenko et al., 2016] contained catalytic part of RPOTmp enzyme with transit peptides of RPOTm (mitochondrial) and RPOTp (plastid) by agrobacterial transformation; plants with complementation of RPOTmp functions in mitochondria or chloroplasts obtained from transformation of GABI_286E07 rpotmp knockout-mutant plants with genetic constructs created in [Tarasenko et al., 2016]. Goal was to determine the effects of RPOTmp knockout/overexpression on global Arabidopsis thaliana gene expression.
2023-12-25 | GSE251661 | GEO
Project description:Comparison of expression differences between Col-0 Arabidopsis thaliana and transgenic plants in the same background carrying three different Fusarium oxysporum effector genes
2017-12-01 | GSE59292 | GEO
Project description:Empty-vector control and transgenic 35S-PaWRKY1 Arabidopsis
| PRJNA128255 | ENA
Project description:Transgenic AhDGR-OE vs Wild type Col 0 Arabidopsis plants
| PRJNA330978 | ENA
Project description:Transgenic Ah24-OE vs Wild type Col 0 Arabidopsis plants
| PRJNA288139 | ENA
Project description:To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. total samples analysed are 24: 4 biological independent replicates of: total leaf (COL-0) vs. enriched guard cells (COL-0); ABA-sprayed enriched guard cells (gl1-1) vs. control-sprayed enriched guard cells (gl1-1); low humidity (20%rh) treated enriched guard cells (COL-0) vs. high humidity (80%) treated enriched guard cells (COL-0)
2013-01-14 | E-GEOD-41054 | biostudies-arrayexpress
Project description:Transgenic AhNF-YC-OE vs Wild type Col 0 Arabidopsis plants
| PRJNA289708 | ENA
Project description:Transgenic Arabidopsis plants with constitutively low inositol (1,4,5) triphosphate exhibit an increased tolerance to water stress by an ABA-independent pathway; The phosphoinositide pathway and inositol (1,4,5) trisphopsphate (InsP3) are implicated in plant responses to stress. In order to manipulate the pathway and determine the downstream consequences of altered InsP3-mediated signaling, we generated transgenic Arabidopsis plants expressing the mammalian type I inositol polyphosphate 5-phosphatase, an enzyme that specifically hydrolyzes the soluble inositol phosphates and terminates the signal. Transgenic plants have no morphological differences compared to wild type; however, rapid transient Ca2+ responses to a cold or salt stimulus are reduced by ~ 30%. To further understand the role of InsP3-mediated signaling in plant stress responses we focused on drought stress. Surprisingly, the InsP 5-ptase plants lose less water and exhibited an increased tolerance to drought. Stomatal bioassays showed that transgenic guard cells are less responsive to the inhibition of opening by ABA but show an increased sensitivity to ABA-induced closure. The onset of the drought stress is delayed in the transgenic plants and ABA levels did not increase as much as in the wild type. Transcript profiling has revealed that DREB2A and a subset of DREB2A regulated genes are basally up regulated in the InsP 5-ptase plants. These results indicate that the drought tolerance of the InsP 5-ptase plants is mediated in part via an ABA-independent pathway. The constitutive dampening of the InsP3 signal in this system has uncovered novel regulation and cross talk between signaling pathways. Experiment Overall Design: In order to compare global expression profiles between wild type and transgenic plants in response to water stress we harvested leaves for transcript profiling at day 0 (well watered) and at day 7 when the transgenic plants were still at an RWC of >85% and the wild type and vector control plants were at an RWC of ~ 50 -60% . Leaves were harvested and pooled from ~ 10 plants of each line/time point and three independent biological replicates were carried out with wild type (WT) and 2 independent transgenic lines (T6 and T8). The vector control line (C2) was used as an additional control.
2008-10-25 | E-GEOD-10670 | biostudies-arrayexpress