Mus musculus
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-6085
PROVIDER: PRJNA97557 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The cytokine IL-2 determines T cell fate by controlling T cell proliferation and differentiation, but the expression files of IL-2 regulated genes are not defined Using Affymetrix mouse genome array and mouse T cell line CTLL-2, we analyzed the expression profiles of IL-2 regulated genes over 10 time points in 24 hours after IL-2 treatment. A total of 2,813 genes were differentially expressed (?2-fold change) at least at one time point in response to IL-2 stimulation. Clustering analyses showed that these genes consist of 9 clusters, suggesting that the IL-2 regulated genes in mouse T cells have 9 expression patterns. These genes involve in many biological processes, including immune response, signal transduction, apoptosis, cell cycle, transcription, transport, biosynthesis and various metabolisms. Two supplementary tables are linked to this series: Table 1: IL-2 responsive genes over the first 24 hours post-stimulation Table 2: Newly identified IL-2 regulated genes function in a variety of biological processes Keywords: stress response
2008-01-11 | GSE6085 | GEO
Project description:The cytokine IL-2 determines T cell fate by controlling T cell proliferation and differentiation, but the expression files of IL-2 regulated genes are not defined; Using Affymetrix mouse genome array and mouse T cell line CTLL-2, we analyzed the expression profiles of IL-2 regulated genes over 10 time points in 24 hours after IL-2 treatment. A total of 2,813 genes were differentially expressed (>=2-fold change) at least at one time point in response to IL-2 stimulation. Clustering analyses showed that these genes consist of 9 clusters, suggesting that the IL-2 regulated genes in mouse T cells have 9 expression patterns. These genes involve in many biological processes, including immune response, signal transduction, apoptosis, cell cycle, transcription, transport, biosynthesis and various metabolisms. Two supplementary tables are linked to this series:; Table 1: IL-2 responsive genes over the first 24 hours post-stimulation; Table 2: Newly identified IL-2 regulated genes function in a variety of biological processes Experiment Overall Design: Murine cytotoxic T lymphocyte cell line CTLL-2, derived from a C57BL/6 mouse, is an IL-2 dependent cell line. Cells were cultured in D10 medium in an incubator with 5% CO2 at 37oC to mid-log phase (~2 X 105 cells/mL), and collected by centrifugation, washed with phosphate buffered saline (PBS) (Invitrogen), resuspended at 1 X 106 cells/mL in the medium without IL-2 (IL-2 starvation) and cultured for 14 hours (No IL-2 stimulation, T0, 5 reps). After adding human rIL-2 (100 U/mL, Chiron, IL-2 stimulation), cells were collected at time point 0.5, 1, 2, 4, 6, 8, 10, 12, 16 and 24 hours, at least 3 biological replicates were carried out for each time point.Total RNA were extracted from the cells and was hybridized on Affymetrix GeneChip Mouse Genome 430 2.0 Array.
2008-11-22 | E-GEOD-6085 | biostudies-arrayexpress
Project description:Proteomic data from dengue virus infected U-937 cells. PVD_C samples were infected via antibody-mediated or DC-SIGN receptor mediated entry routes with wild-type DENV-4 or mock inoculum; time points 2, 8, 16, and 24 hours; 5 biological replicates. PVD_L samples were infected via antibody-mediated or DC-SIGN receptor mediated entry routes with wild-type DENV-1 or mock inoculum; time points 2, 6, 10, 18, 24, and 30 hours; 5 biological replicates.
2019-08-22 | PXD014780 | Pride
Project description:IL-36 cytokines have recently emerged as mediators of inflammation in autoimmune conditions including psoriasis vulgaris (PsV) and generalized pustular psoriasis (GPP). This study used RNA-seq to profile the transcriptome of primary epidermal keratinocytes (KCs) treated with IL-1B, IL-36A, IL-36B or IL-36G. We identified some early IL-1B-specific responses (8 hours post-treatment), but nearly all late IL-1B responses were replicated by IL-36 cytokines (24 hours post-treatment). Type I and II interferon genes exhibited time-dependent response patterns, with early induction (8 hours) followed by no response or repression (24 hours). Altogether, we identified 225 differentially expressed genes (DEGs) with shared responses to all 4 cytokines at both time points (8 + 24 hours). These involved up-regulation of ligands (IL1A, IL1B, IL36G) and activating proteases (CTSS), but also up-regulation of inhibitors such as IL1RN and IL36RN. Shared IL-1B/IL-36 DEGs overlapped significantly with genes altered in PsV and GPP skin lesions, as well as genes near GWAS loci linked to autoimmune and autoinflammatory diseases (e.g., PsV, psoriatic arthritis, IBD, primary biliary cholangitis). Inactivation of MyD88 adapter protein using CRISPR/Cas9 completely abolished expression responses of such DEGs to IL-1B and IL-36G stimulation. These results provide a global view of IL-1B and IL-36 expression responses in epidermal KCs with fine-scale characterization of time-dependent and cytokine-specific response patterns. Our findings support an important role for IL-1B and IL-36 in autoimmune or autoinflammatory conditions and show that MyD88 adaptor protein mediates shared IL-1B/IL-36 responses.
2018-03-04 | GSE109182 | GEO
Project description:Comparing control and LPS-administered with either 40 mg/kg LPS for early time points (ETP) or 10 mg/kg for late time points (LTP). In ETP, 7-7 animals were sacrificed at 1.5 and at 6 hours after LPS administration. In LTP, 7-7 animals were sacrificed at 24 and 48 hours after the endotoxin injection. In both ETP and LTP 7 mice were received equal volume of saline to use as negative control. Four animals were selected for miRNA microArray analysis based on their proinflammatory (TNF-α and IL-6) mRNA expression levels.
2019-11-06 | GSE139919 | GEO
Project description:In the context of studying visceral leishmaniasis, neutrophils infected with Leishmania donovani have been compared to uninfected neutrophils. Compared time points are 0, 6 and 24 hours post infection. Neutrophils of three human donors have been used. Overall 6 samples for infected neutrophils at time point 6 hours and 6 samples for infected neutrophils at time point 24 hours exist, including three biological samples and two technical samples. Uninfected neutrophils represent 3 samples at time point 0 hours, 3 samples at time point 6 hours and 3 samples at time point 24 hours. Transcriptome of Leishmania donovani culture has been assessed in two replicates.
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Project description:Murine ES cell gene expression before RA induction are used to compare gene expression for time-points of 8, 12, 16, 24, 36, 48, 60 and 72 hours post-induction.
2012-12-20 | GSE34279 | GEO
Project description:Analysis of gene expression in injured primary DRG with or without camptothecin (CPT) treatment after sciatic nerve crushing may help us identify critical molecular pathways related to axon regeneration. We performed RNA-sequencing of (i) Naive primary DRG tissues without injury, (ii) Primary DRG tissues with vehicle treatment different time-points (18, 24, 36 hours) after sciatic nerve injury, and (iii) Primary DRG tissues with camptothecin treatment different time-points (18, 24, 36 hours) after sciatic nerve injury.
2020-07-24 | GSE113672 | GEO
Project description:RNA-seq of 24 M-CSF differentiated human peripheral monocyte-derived macrophages (MDMs) activated with short exposure (3hours) to LPS, or long exposure (24 hours) to LPS, LPS with IFNγ, IFNγ, IL-4, IL-10, and dexamethasone.
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Project description:Gene expression profiling of elutriated monocytes cultured for 48 hours in RPMI-1640, 10% AB plamsa and one of the following: GMCSF (56IU/ml), IL-4 (2000 IU/ml), 15 kDa Granulysin (10 nM) Sources tested: monocytes form 3 donors tested at time 0, 4 hours, 12 hours, 24 hours, 48 hours 5 time points were compared: day 0 (cells before start the culture), 4, 12, 24, 48 hours
2012-05-18 | E-GEOD-35304 | biostudies-arrayexpress