Project description:Peripheral blood was collected from human patients infected with influenza A virus only or in addition with bacterial pathogens and at different days after hospital admission. For comparison, blood from healthy controls was collected. Gene expression differences were detected in influenza and bacterial infections compared to healthy controls, and at various days post infection.
Project description:We focused on the major peripheral blood lymphocyte populations that may be involved in anti-tumor responses and negatively impacted by cancer, specifically CD8 T cells, CD4 T cells, B cells and CD56dim natural killer cells. The pure cell subsets were stringently sorted by flow cytometry from PBMC samples. Gene expression profiles of these cell populations from melanoma patients were compared to healthy controls. Keywords: gene expression profile
Project description:The samples was divided in to three groups including the T cells extracted from the tumor infiltrating lymphocytes and from the paired peripheral blood bymphocyte of 3 patients sufferring from hepatocellular carcinoma as well as the peripheral blood bymphocyte of three healthy controls. The total RNA was extracted for the detection of human lncRNA expression level.
Project description:Total transcriptome and gene expression profile of peripheral blood mononuclear cells (PMBCs) from 5 patients with CAD and 5 healthy controls were analyzed by RNA sequencing.
Project description:The objective of this experiment was to compare the transcriptomic profile (NanoString platform) of peripheral blood mononuclear cells (PBMCs) from COVID-19 patients with mild disease, and patients with severe COVID-19 with and without dexamethasone treatment, and healthy controls. We analyzed PBMCs from 4 mild COVID patients, 3 severe COVID patients,4 severe COVID patients treated with dexamethasone, and 5 healthy controls
Project description:Aberrant gene expression analysis between peripheral blood mononuclear cells (PBMCs) samples from healthy controls (HC) and patients with systemic lupus erythmatosus (SLE) were identified using Affymetrix gene arrays
Project description:CD8+ T cells are the primary target of immune checkpoint inhibitor (ICI) therapy in the treatment of melanoma. ICI therapy only benefits a subset of patients and complicating this issue is a reliable prediction method that does not require invasive biopsies. In the hope of remedying this challenge, we conducted single-cell transcriptomic analyses of CD8+ T cells in peripheral blood lymphocytes (CD8-mPBLs) and, importantly, tumor-infiltrating lymphocytes (CD8-mTILs) from 8 patients with metastatic melanoma.
