Project description:Expression profiles of HeLa CD4+ cells transfected with epitope-tagged eTat plasmid, or parental plasmid pCep4. Cells are unsynchronized and untreated.
Project description:Expression profiles of HeLa CD4+ cells transfected with epitope-tagged eTat plasmid, or parental plasmid pCep4. Cells are unsynchronized and untreated. Keywords: other
Project description:Expression profiles of HeLa CD4+ cells transfected with epitope-tagged eTat plasmid. Cells are treated with nocodazole and hydroxyurea. Keywords: time-course
Project description:Expression profiles of HeLa CD4+ cells transfected with epitope-tagged eTat plasmid. Cells are treated with nocodazole and hydroxyurea.
Project description:The distribution of histone variants H2Abbd and macroH2A in 13 regions of the HG18 assembly have been studied using a variant of the ChIP-on-Chip technique. HeLa S3 cell lines expressing tagged histones H2A, H2Abbd or macroHA were obtained using retroviral transfer. DNA fractions associated with tagged histones were isolated using a two-step purification procedure that involved affinity chromatography on a column with anti-FLAG antibodies, followed by affinity chromatography on a Ni-agarose column. The obtained genomic DNA samples were analyzed by hybridization with custom NimbleGene genomic microarrays. Two samples. Test sample 1 is HeLa S3 cells expressing epitope-tagged histone H2Abbd and test sample 2 is HeLa S3 cells expressing epitope-tagged histone macroH2A . The control for both test sample 1 and test sample 2 is HeLa S3 expressing epitope-tagged histone H2A. Two copies of each probe per array were made.
Project description:Expression profiles of HeLa CD4+ cells transfected with parental vector pCep4. Cells are treated with nocodazole and hydroxyurea. Keywords: time-course
Project description:To identify direct transcriptional targets of RFX6, we performed chromatin immunoprecipitation of HA epitope tagged RFX6 followed by massively parallel DNA sequencing (ChIP-seq). Using CRISPR/Cas9 gene editing, the HA epitope was inserted into the 3' end of the RFX6 gene in H9 hESC. Pluripotent cells were then differentiated into PDX1+RFX6+ pancreatic progenitors and endogenous RFX6-HA was immunoprecipitated with an anti-HA antibody. To eliminate background signal caused by non-specific antibody binding, a control experiment using wild-type H9 hESC was performed in parallel.
Project description:The aim of this study was initially to determine the mechanism by which SARM1 regulates Ccl5 expression in murine macrophages. RNA sequencing revealed that the commonly used Sarm1tm1Aidi mouse, generated by targeted gene disruption, harbours passenger genes on chromosome 11 which confound interpretation of differential gene expression. Next-generation SARM1-deficient and epitope-tagged mice generated by CRISPR/Cas9 reveal no broad role for SARM1 in transcription in macrophages or brainstem, despite SARM1 protein expression there.