Project description:This SuperSeries is composed of the following subset Series: GSE106: OVX/P4/UTX 129/SvImJ WT A; GSE107: OVX/P4/UTX 129/SvImJ WT B Experiment Overall Design: Refer to individual Series. Female mice used were 129/SvImJ wildtype (Jackson Lab), 10-16 weeks of age. 14 days after ovariectomy, mice were injected subcutaneously with progesterone dissolved in sesame oil (Sigma; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine horns at various times after injection. 8 uterine horns from 4 mice were pooled to form one sample. Total RNA preparation, cRNA labeling and hybridization were performed according to recommendations by Affymetrix.
Project description:Female mice used were 129/SvImJ wildtype (Jackson Lab), 10-16 weeks; of age. 14 days after ovariectomy, mice were injected; subcutaneously with progesterone dissolved in sesame oil (Sigma;; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine; horns at various times after injection. 8 uterine horns from 4 mice were; pooled to form one sample. Total RNA preparation, cRNA labeling and; hybridization were performed according to recommendations by; Affymetrix.
Project description:This SuperSeries is composed of the following subset Series:; GSE108: OVX/P4/6h/UTX 129 WT vs. Hoxa-10-/-; GSE109: OVX/oil/6h/UTX 129 WT vs. Hoxa-10-/- Experiment Overall Design: Refer to individual Series. Female mice used were SvJ/129, 10-16 weeks of age; Hoxa-10+/+ and Hoxa-10-/- littermates were used in parallel experiments. 14 days after ovariectomy, mice were injected subcutaneously with progesterone dissolved in sesame oil (Sigma; 0.2mg/0.1mL/mouse) or sesame oil (Sigma; 0.1mL/mouse), followed by sacrifice and removal of uterine horns at 6 hours after injection. 8 uterine horns from 4 mice were pooled to form one sample. Total RNA preparation, cRNA labeling and hybridization were performed according to recommendations by Affymetrix.
Project description:Female mice used were 129/SvImJ wildtype (Jackson Lab), 10-16 weeks; of age. 14 days after ovariectomy, mice were injected; subcutaneously with progesterone dissolved in sesame oil (Sigma;; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine; horns at various times after injection. 8 uterine horns from 4 mice were; pooled to form one sample. Total RNA preparation, cRNA labeling and; hybridization were performed according to recommendations by; Affymetrix.
