Dataset Information

In vitro phosphorylation of ASY1 by CDKA;1

ABSTRACT: Meiosis is a complex cell division program reducing chromosome number as a prerequisite for sexual reproduction and at the same time leading to a new combination of parental alleles contributing to biodiversity. Little is known about how the distinct order of the molecular processes of meiosis are orchestrated. Here we show that the Arabidopsis Cdk1/Cdk2 homolog CDKA;1 is a master regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis Hop1 homolog, which is required for synaptonemal complex formation, as a phospho-target of CDKA;1. Live cell imaging together with biochemical assays shows that CDK-dependent phosphorylation of ASY1 is required for its recruitment to the chromosome axis via ASYNAPTIC 3 (ASY3), the Arabidopsis Red1 homolog, counteracting the disassembly activity of the AAA+ ATPase PACHYTENE CHECKPOINT 2 (PCH2). Furthermore, we have mapped the closure motif of ASY1, typical for HORMA domain proteins, and provide evidence that the Cdk-dependent phosphorylation of ASY1 regulates the self-polymerization of ASY1 along the chromosome axis during early meiosis. Hence, the phosphorylation of ASY1 by CDKA;1 appears to be a two-pronged mechanism to initiate chromosome axis formation in meiosis.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Sara Stolze

PROVIDER: PXD011335 | panorama | Tue Oct 01 00:00:00 BST 2019

REPOSITORIES: PanoramaPublic

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Publications

The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis.

Yang Chao C Sofroni Kostika K Wijnker Erik E Hamamura Yuki Y Carstens Lena L Harashima Hirofumi H Stolze Sara Christina SC Vezon Daniel D Chelysheva Liudmila L Orban-Nemeth Zsuzsanna Z Pochon Gaëtan G Nakagami Hirofumi H Schlögelhofer Peter P Grelon Mathilde M Schnittger Arp A

The EMBO journal 20190926 3

Meiosis is key to sexual reproduction and genetic diversity. Here, we show that the Arabidopsis cyclin-dependent kinase Cdk1/Cdk2 homolog CDKA;1 is an important regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis homolog of Hop1 (homolog pairing 1), essential for synaptonemal complex formation, as a target of CDKA;1. The phosphorylation of ASY1 is required for its recruitment to the ch ...[more]

PMID: 31556459

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Project description:Transcriptional profiling of age-related change of callus formation capability in Arabidopsis hypocotyls Organogenesis in vitro consists of many aspects such as phytohormone perception, dedifferentiation of differentiated cell to acquire organogenic competence, and re-entry of quiescent cells into cell cycle. In this study, we established an in vitro experimental system to study the age-dependent callus formation capacity in Arabidopsis. Interestingly, mature (35- to 38-day-old) hypocotyl explants exhibited better callus-forming potential than that of juvenile (7- to 10-day-old), determined by callus growth rates. To explore genome-wide expression changes underlying the phenomenon of age-dependent callus formation, a transcriptome-based analysis was performed. Gene expression profiling indicated that age-dependent callus formation capacity was associated with changes in phytohormone (auxins, cytokinins, abscisic acid, brassinosteroids and gibberellins) homeostasis, epigenetic mechanism and the cell cycle regulation. Besides, we identified two groups of genes involved in age-dependent callus formation capacity: (1) positive regulatory and (2) negative regulatory categories, i.e. genes that were significantly up- or down-regulated during callus formation derived from mature explants, respectively. One gene encoding DNA-binding protein (VARIANT IN METHYLATION 1, VIM1) belonging to the positive regulatory category was selected for functional analysis and assessment of age-dependent callus formation capacity. Indeed, vim1 reduced the efficiency of callus formation in mature explants, but not in juvenile. The result suggests that VIM1 plays an important role in regulating age-dependent callus formation capacity. Taken together, the investigation will help to better understand the molecular regulatory mechanism of age-dependent callus formation. Comparison of young and mature Arabidopsis hypocotyls either with or without auxin treatment for 1 day

Dataset Information

In vitro phosphorylation of ASY1 by CDKA;1

Publications

The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis.

Similar Datasets

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