Exploring Non-Canonical Terpene Space With a Heterologous Lepidopteran Mevalonate Pathway

ABSTRACT: Lepidoptera (butterflies and moths) make the six-carbon compounds homoisopentenyl pyrophosphate (HIPP) and homodimethylallyl pyrophosphate (HDMAPP) that are incorporated into sixteen, seventeen and eighteen carbon farnesyl pyrophosphate (FPP) analogues. In this work we heterologously expressed the lepidopteran modified mevalonate pathway, a propionyl-CoA ligase, and terpene cyclases in E. coli to produce several novel terpenes containing sixteen carbons. Changing the terpene cyclase generated different novel terpene product profiles. To further validate the new compounds we confirmed 13C propionate was incorporated, and that the masses and fragmentation patterns were consistent with novel sixteen carbon terpenes by GC-QTOF. Based on the available FPP analogues lepidoptera produce, this approach should greatly expand the reachable biochemical space with applications in areas where terpenes have traditionally found uses.

ORGANISM(S): Escherichia Coli

SUBMITTER: Chris Petzold  

PROVIDER: PXD012697 | panorama | Tue Oct 29 00:00:00 GMT 2019

REPOSITORIES: PanoramaPublic