Dataset Information

Improving peptide fragment ion detection using travelling wave ion mobility spectrometry with signal enhancement MSE (seMSE)

ABSTRACT: Recently, a solution to inherent poor ion beam sampling of time-of-flight (TOF) mass analyzers was proposed by restricting detection to ions expected at specific arrival times after being separated in a travelling wave ion mobility spectrometry (TWIMS) cell. Here, we show that this approach can be applied to data independent acquisition (DIA) using a so-called signal enhancement MSE (seMSE) experiment, which was compared to two different TWIMS-DDA and three different TWIMS-MSE methods with respect to the number of peptide identifications, the spectral quality of supporting peptide spec-tra matches, and, more importantly, fragment ion sensitivity. Comparison of fragment ion summed areas showed that seM-SE showed 6.8 to 11.5-fold improvement in fragment ion detection when compared to other MSE methods and although co-fragmentation of co-eluting peptides limited the seMSE peptide identifications it was possible to consistently detect peptides of interest by integrating DDA and MSE results within Skyline.

ORGANISM(S): Oryctolagus Cuniculus Escherichia Coli Bos Taurus Saccharomyces Cerevisiae

SUBMITTER: Juan Camilo Rojas Echeverri

PROVIDER: PXD030431 | panorama | Mon Aug 01 00:00:00 BST 2022

REPOSITORIES: PanoramaPublic

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Publications

Evaluating Peptide Fragment Ion Detection Using Traveling Wave Ion Mobility Spectrometry with Signal-Enhanced MSE (SEMSE).

Rojas Echeverri Juan Camilo JC Volke Daniela D Milkovska-Stamenova Sanja S Hoffmann Ralf R

Analytical chemistry 20220729 31

The inherent poor sampling of fragment ions in time-of-flight mass analyzers was recently improved for data-dependent acquisition (DDA) by considering their drift times in traveling wave ion mobility spectrometry (TWIMS). Here, we extend this TWIMS-DDA approach to the data-independent acquisition (DIA) mode MSE to improve the signal intensities of fragment ions by providing improved ion beam sampling efficiency, which we termed therefore signal-enhanced MSE (SEMSE</sup ...[more]

PMID: 35904512

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Dataset Information

Improving peptide fragment ion detection using travelling wave ion mobility spectrometry with signal enhancement MSE (seMSE)

Publications

Evaluating Peptide Fragment Ion Detection Using Traveling Wave Ion Mobility Spectrometry with Signal-Enhanced MS<sup>E</sup> (SEMS<sup>E</sup>).

Similar Datasets

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