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Validation of known and novel AKT, S6K and RSK targets by targeted MS

ABSTRACT: Using the global SILAC and label-free phosphoproteomics experiments as a basis, an inclusion list for validation of known and novel AKT, RSK and S6K targets comprising the RxRxxp[ST] motif was generated. Samples were analyzed by PRM-MS.

ORGANISM(S): Mus Musculus

SUBMITTER: Anna Lena Fricke

PROVIDER: PXD035962 | panorama | Mon Feb 27 00:00:00 GMT 2023

REPOSITORIES: PanoramaPublic

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Phosphoproteomics Profiling Defines a Target Landscape of the Basophilic Protein Kinases AKT, S6K, and RSK in Skeletal Myotubes.

Fricke Anna L AL Mühlhäuser Wignand W D WWD Reimann Lena L Zimmermann Johannes P JP Reichenbach Christa C Knapp Bettina B Peikert Christian D CD Heberle Alexander M AM Faessler Erik E Schäuble Sascha S Hahn Udo U Thedieck Kathrin K Radziwill Gerald G Warscheid Bettina B

Journal of proteome research 20230210 3

Phosphorylation-dependent signal transduction plays an important role in regulating the functions and fate of skeletal muscle cells. Central players in the phospho-signaling network are the protein kinases AKT, S6K, and RSK as part of the PI3K-AKT-mTOR-S6K and RAF-MEK-ERK-RSK pathways. However, despite their functional importance, knowledge about their specific targets is incomplete because these kinases share the same basophilic substrate motif RxRxx<i>p</i>[ST]. To address this, we performed a ...[more]

PMID: 36763541

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