Dynamic Data Independent Acquisition Mass Spectrometry with Real-Time Retrospective Alignment

ABSTRACT: We report a data independent acquisition (DIA) strategy that dynamically adjusts the tandem mass spectrometry (MS/MS) windows during the chromatographic separation. The method focuses MS/MS acquisition on the most relevant mass range at each point in time – improving the quantitative sensitivity by increasing the time spent on each DIA window. We demonstrate an improved lower limit of quantification, on average, without sacrificing the number of peptides detected.

ORGANISM(S): Homo Sapiens

SUBMITTER: Lilian Heil  

PROVIDER: PXD038508 | panorama | Mon Sep 18 00:00:00 GMT+01:00 2023

REPOSITORIES: PanoramaPublic