Proteomics

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Sensitive detection of HIV-1 and Mycobacterium tuberculosis peptides in blood for rapid diagnosis of co-infected individuals by immuno-affinity liquid chromatography tandem mass spectrometry

ABSTRACT: We describe a blood-based immuno-affinity liquid chromatography tandem mass spectrometry (ILM) assay that quantifies proteotypic peptides derived from the Mtb virulence factor CFP10 and the HIV-1 capsid protein p24 to simultaneously diagnose HIV-1 infection and TB disease and to monitor their real-time response to treatment.

ORGANISM(S): Homo Sapiens

SUBMITTER: Lin Li  

PROVIDER: PXD043191 | panorama | Wed Nov 15 00:00:00 GMT 2023

REPOSITORIES: PanoramaPublic

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Sensitive Blood-Based Detection of HIV-1 and Mycobacterium tuberculosis Peptides for Disease Diagnosis by Immuno-Affinity Liquid Chromatography-Tandem Mass Spectrometry: A Method Development and Proof-of-Concept Study.

Li Lin L   Lyon Christopher J CJ   LaCourse Sylvia M SM   Zheng Wenshu W   Stern Joshua J   Escudero Jaclyn N JN   Murithi Wilfred Bundi WB   Njagi Lilian L   John-Stewart Grace G   Hawn Thomas R TR   Nduba Videlis V   Abdelgaliel Wael W   Tombler Thomas T   Horne David D   Jiang Li L   Hu Tony Y TY  

Clinical chemistry 20231201 12

<h4>Background</h4>Novel approaches that allow early diagnosis and treatment monitoring of both human immunodeficiency virus-1 (HIV-1) and tuberculosis disease (TB) are essential to improve patient outcomes.<h4>Methods</h4>We developed and validated an immuno-affinity liquid chromatography-tandem mass spectrometry (ILM) assay that simultaneously quantifies single peptides derived from HIV-1 p24 and Mycobacterium tuberculosis (Mtb) 10-kDa culture filtrate protein (CFP10) in trypsin-digested serum  ...[more]

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