Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In-Gel digest and filter-aided sample preparation to identify Dermatophagoides - and yeast protein antigens in severe equine asthma.

ABSTRACT: Severe equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans. A 2D immunoblot approach was used to identify quantitative differences in the recognition of the two-dimensional gel electrophoresis (2-DE) pattern between asthmatic and helthy horses´sera. Differentially regulated spots were cut from a 2-D gel and used for in-gel digestion followed by LC-MS to identify possible antigens.

ORGANISM(S): Dermatophagoides Pteronyssinus Saccharomyces Cerevisiae

SUBMITTER: Daniela Volke

PROVIDER: PXD045568 | panorama | Fri Jan 26 00:00:00 GMT 2024

REPOSITORIES: PanoramaPublic

ACCESS DATA

Json Xml

Publications

Immunoproteomics reveal increased serum IgG3/5 binding to <i>Dermatophagoides</i> and yeast protein antigens in severe equine asthma in a preliminary study.

Schnabel Christiane L CL Jentsch Maria-Christin MC Lübke Sabrina S Kaiser-Thom Sarah S Gerber Vinzenz V Vrtala Susanne S Huang Huey-Jy HJ Rhyner Claudio C Wagner Bettina B Hoffmann Ralf R Volke Daniela D

Frontiers in immunology 20231215

<h4>Introduction</h4>Severe equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans. SEA-provoking antigens have not been comprehensively characterized, but molds and mites have been suggested as relevant sources. Here, we identified relevant antigen candidates using immunoproteomics with IgG isotype-binding analyses.<h4>Methods</h4>Proteins from <i>Dermatophagoides pte ...[more]

PMID: 38162673

Similar Datasets

Analysis of Chromosome VIII of EC-C1 strains (S. cerevisiae)

Project description:The genomic DNA of rearranged chromosome VIII (~900kb) of EC9 diploid strain was extracted from the gel of pulsed field gel electrophoresis (PFGE), and analyzed by array-CGH to identify it's amplified regions

2012-05-18 | GSE38034 | GEO

Analysing of Chromosome VIII of EC9-7 after experimental evolution (S. cerevisiae)

Project description:The genomic DNA of wild-type chromosome VIII of evolved EC9-7 cells was extracted from the gel of pulsed field gel electrophoresis (PFGE), and analyzed by array-CGH to identify its chromosomal composition

2011-11-13 | GSE33652 | GEO

Gel-free library preparation for next-generation RNA sequencing and small RNA quantification

Project description:Next-generation RNA sequencing (RNA-seq) is hampered by “primer dimer” (PD) artifacts and its quantitative performance reduced by polymerase fall-off (PF) at RNA modifications and secondary structures. Here we improved RNA-seq efficiency by incorporating (i) a post-reverse-transcription (RT) digestion of excess primers with Escherichia coli exonuclease I for PD mitigation, thus obviating gel purification during RNA-seq library preparation, and (ii) a high-processivity reverse transcriptase to increase full-length reads. A full factorial experimental design was applied to absolute quantification RNA sequencing (AQRNA-seq), the most accurate NGS-based method for quantifying small RNAs, using cDNA libraries constructed from E. coli small RNAs (>85% tRNA) followed by sequencing, data processing, and data analysis. The novel PF and PD mitigation approaches increased AQRNA-seq sensitivity >10-fold by minimizing PF and maximizing target RNA reads. By increasing sensitivity and obviating gel electrophoresis for removing PD, AQRNA-seq and other NGS-based RNA-seq methods can now be automated to increase throughput and reduce RNA sample size.

2025-12-15 | GSE313501 | GEO

Immunoproteomic analysis of the excretory-secretory products of the Trichinella pseudospiralis adult worms and newborn larvae

Project description:In this study, we used Two-dimensional gel electrophoresis (2-DE)-based Western blotting coupled with MALDI-TOF / TOF-MS / MS to separate and identify the T. pseudospiralis adult worms (Ad) ES products immunoreaction-positive proteins. In total, 400 protein spots were separated by 2-DE. 28 protein spots were successfully identified using the sera from infected pigs and were characterized to correlate with twelve different proteins of T. pseudospiralis.

2018-11-22 | PXD007783 | Pride

Comparative proteome analysis of a clonal virulent and attenuated Histomonas meleagridis strain.

Project description:The current study aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which was in vitro co-cultivated with Escherichia coli DH5α. Two-dimensional gel electrophoresis (2-DE) was used for proteome visualization , gel image software for computational detection of significantly up-regulated protein spots and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) for protein identification. The statistical analysis fulfilling two criteria (> or = 3-fold up-regulation and P<0.05) detected 119 differentially expressed protein spots out of which 62 spots were located in gels of the virulent strain and 57 spots in gels of the attenuated strain. The mass spectrometric analysis of 32 spots, up-regulated in gels of the virulent strain, showed that they are of H. meleagridis origin. As opposed to this, the mass spectrometric analysis of 49 protein spots , up-regulated in the gels of the attenuated strain , identified 32 spots as specific to the protozoan. Additionally, the analysis identified a number of E. coli DH5α proteins which were detected as differentially expressed by the computational gel image and statistical analysis.

2017-12-07 | PXD007579 | Pride

In-Gel Digest to identify Aspergillus fumigatus protein antigens in severe equine asthma (SEA)

Project description:Aspergillus fumigatus (Asp f) has been described as a major extrinsic stimulus of SEA and is a common mold species in hay. Aiming to identify disease-relevant antigens, we analyzed Asp f as a source, in an immuno-proteomics approach on two-dimensional immunoblots. For 21 of 289 spots serum Ig binding was different between asthmatic and healthy horses’ sera. Proteins were in-gel digested and tryptic peptides extracted from the 21 spots of interest, analyzed by liquid chromatography-mass spectrometry and eight identified and prioritized candidate antigens were expressed as recombinant proteins.

2024-04-29 | PXD045869 | panorama

Thylakoids of Picea abies - The unique photosynthetic apparatus of Pinaceae - Analysis of photosynthetic complexes in Norway spruce (Piceaabies)

Project description:Analysis of the subunits composition of the thylakoids protein complexes in Picea abies (Norway spruce) by means of two-dimensional large-pore Blue-Native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D lpBN/SDS-PAGE) and in-gel tryptic digestion of single spots.

2019-04-02 | PXD010071 | Pride

Analysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS

Project description:Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel digestion and quantitative LC-MS/MS. Totally 2552 proteins were assigned from the lane of supernatant and 2614 protein species from the lane of precipitate. The results are being further analyzed, e.g. 1) for the comparison between the proteins species residing in the two fractions, and 2) for correlation with the nondenaturing 2DE-grid cut-quantitative LC-MS/MS results (Jin, et al. Electrophoresis 2015, 36, 1711-1723, Jin, et al. Electrophoresis 2015, 1991-2001, PRIDE project PXD001471).

2019-01-03 | PXD010036 | Pride

Sample Preparation and Imaging Conditions Affect mEos3.2 Photophysics in Fission Yeast Cells.

Project description: Not available

| S-EPMC7820738 | biostudies-literature

Proteomic analysis of seed germination of wheat (Triticum aestivum L.)

Project description:In this study, we performed the first dynamic proteome analysis of wheat seed germination using a two-dimensional differential gel electrophoresis (2D-DIGE)-based proteomic approach. A total of 166 differentially expressed protein (DEP) spots representing 73 unique proteins were identified.

2015-12-09 | PXD003083 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data