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Stable Isotope Labeling by Amino acid in Vivo (SILAV)

ABSTRACT: Intravenous administration of stable isotope labeled amino acid ((13)C6-leucine) to humans recently made it possible to study the metabolism of specific biomarkers in cerebrospinal fluid (CSF) and in plasma using targeted mass spectrometry (MS). This labeling approach could be of great interest for monitoring many leucine-containing peptides in parallel, using Mass Spectrometry. This will make it possible to quantify the rates of synthesis and clearance of a large range of proteins in humans with a view to obtaining new insights into protein metabolism processes and the pathophysiology of diseases such as Alzheimer's disease.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jerome VIALARET

PROVIDER: PXD046142 | panorama | Sat Nov 15 00:00:00 GMT 2025

REPOSITORIES: PanoramaPublic

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Modeling the Simultaneous Dynamics of Proteins in Blood Plasma and the Cerebrospinal Fluid in Human <i>In Vivo</i>.

Giroux Pierre P Vialaret Jérôme J Kindermans Jana J Gabelle Audrey A Bauchet Luc L Hirtz Christophe C Lehmann Sylvain S Colinge Jacques J

Journal of proteome research 20240610 7

The analysis of protein dynamics or turnover in patients has the potential to reveal altered protein recycling, such as in Alzheimer's disease, and to provide informative data regarding drug efficacy or certain biological processes. The observed protein dynamics in a solid tissue or a fluid is the net result of not only protein synthesis and degradation but also transport across biological compartments. We report an accurate 3-biological compartment model able to simultaneously account for the p ...[more]

PMID: 38857467

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Stable Isotope Labeling by Amino acid in Vivo (SILAV)

Project description:Intravenous administration of stable isotope labeled amino acid ((13)C6-leucine) to humans recently made it possible to study the metabolism of specific biomarkers in plasma using High Resolution mass spectrometry (MS). This labeling approach could be of great interest for monitoring many leucine-containing peptides in parallel, using HRMS. This will make it possible to quantify the rates of synthesis and clearance of a large range of proteins in humans with a view to obtaining new insights into protein metabolism processes and the pathophysiology of diseases such as Alzheimer's disease.

2025-11-10 | PXD046373 | panorama

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