Dataset Information

Establishing personalized blood protein reference ranges using non-invasive microsampling and targeted proteomics: Implications for anti-doping strategies

ABSTRACT: To prevent doping practices in sports, the World Anti-Doping Agency implemented the Athlete Biological Passport (ABP) program, monitoring biological variables over time to indirectly reveal effects of doping rather than detect the doping substance or method itself. In the context of this program and as part of the Partnership for Clean Competition, we developed a highly multiplexed mass spectrometry-based proteomics assay for 319 peptides corresponding to 250 proteins, including proteins associated with blood doping practices. We determined the ‘baseline’ expression profiles of these potential biomarkers in capillary blood (dried blood spots, DBS) using multiple reaction monitoring (MRM). Combining DBS micro-sampling with highly multiplexed MRM assays is the best suited technology to enhance the effectiveness of the ABP program, as it represents a cost-effective and robust alternative analytical method with high specificity and selectivity of targets in the attomole range. DBS were collected from 10 healthy athletes over a period of 140 days (28 time points per participant). These comprehensive findings provide a personalized blood proteome "fingerprint" showcasing that the proteome is unique to an individual, and likely comparable to a DNA-fingerprint. The results can serve as a baseline for future studies investigating doping-related perturbations.

ORGANISM(S): Homo Sapiens

SUBMITTER: Vincent Richard

PROVIDER: PXD048168 | panorama | Wed Aug 13 00:00:00 GMT+01:00 2025

REPOSITORIES: PanoramaPublic

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Establishing Personalized Blood Protein Reference Ranges Using Noninvasive Microsampling and Targeted Proteomics: Implications for Antidoping Strategies.

Richard Vincent R VR Mitsa Georgia G Eshghi Azad A Chaplygina Daria D Mohammed Yassene Y Goodlett David R DR Zahedi Rene P RP Thevis Mario M Borchers Christoph H CH

Journal of proteome research 20240424 5

To prevent doping practices in sports, the World Anti-Doping Agency implemented the Athlete Biological Passport (ABP) program, monitoring biological variables over time to indirectly reveal the effects of doping rather than detect the doping substance or the method itself. In the context of this program, a highly multiplexed mass spectrometry-based proteomics assay for 319 peptides corresponding to 250 proteins was developed, including proteins associated with blood-doping practices. "Baseline" ...[more]

PMID: 38655860

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Project description:The Malaria Host-Pathogen Interaction Center (MaHPIC) is an inter-disciplinary NIH systems biology contract that utilizes multiple omics types (omics, clinical, and mathematical models), to iteratively test and develop hypotheses related to the complex host-parasite dynamics in the course of malaria infections in non-human primates with the goal of better understanding human disease. These data and metadata were produced by MaHPIC in collaborations between Emory University, Georgia Institute of Technology, and The University of Georgia as part of the MaHPIC contract (NIAID Contract: # HHSN272201200031C). Curation and maintenance of these data and metadata are the responsibility of the MaHPIC. Mary Galinski mgalins@emory.edu (MaHPIC Program Director), Jessica Kissinger jkissinger@uga.edu (MaHPIC Co-Program Director), Esmeralda Meyer evargas@emory.edu - MaHPIC Project Manager The experimental design of this pyrimethamine administration experiment with Macaca mulatta was approved by the Emory University Institutional Animal Care and Use Committee (IACUC) and is as follows. Five naive males (RCs13, RWr13, RUn13, RZe13 and RTi13) approximately 2 years of age were injected intravenously with a preparation of Anopheles dirus salivary gland material and then profiled for clinical and omic measurements over the course of a 100-day experiment. Samples were generated and analyzed as part of a multi-omic approach to understanding the effects that the anti-malarial drug pyrimethamine has on immune physiology in rhesus macaques (M. mulatta). There was no infection with Plasmodium parasites during this study. Whole blood and bone marrow RNA-Seq and plasma have been obtained for seven time-points before, during and after three rounds of drug administration. Samples are from either whole blood or bone marrow specimen types. 5 individuals were sampled for each specimen type, 7 times each over the 100 day experiment. 5 individuals * 2 specimen types * 7 time points = 70 samples. Biological samples (Whole Blood or Bone Marrow) were collected at 7 time points during the 100 days. These correspond to: Time Point 1 = Day 0 (Baseline Sampling Point) Time Point 2 = Day 21 Time Point 3 = Day 27 Time Point 4 = Day 52 Time Point 5 = Day 59 Time Point 6 = Day 90 Time Point 7 = Day 98 Pyrimethamine was administered on Days 21, 52, 53, 54, 90, 91, and 92.

Dataset Information

Establishing personalized blood protein reference ranges using non-invasive microsampling and targeted proteomics: Implications for anti-doping strategies

Publications

Establishing Personalized Blood Protein Reference Ranges Using Noninvasive Microsampling and Targeted Proteomics: Implications for Antidoping Strategies.

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