Dataset Information

Targeted mass spectrum on activation-state-dependent Integrin alpha 5

ABSTRACT: Integrin alpha 5 is a key transmembrane protein that mediates cell-ECM adhesion and ECM assembly. Its activation and signaling involve both cytoplasmic and extracellular proteins. Using zebrafish somitogenesis as the physiological setting, we sought to identify activation-state-dependent Integrin alpha 5 (Itga5) associated proteins.

ORGANISM(S): Danio Rerio

SUBMITTER: Guangyu Sun

PROVIDER: PXD058747 | panorama | Mon Nov 10 00:00:00 GMT 2025

REPOSITORIES: PanoramaPublic

ACCESS DATA

Publications

Actn4 links inactive Integrin α5 with actin in zebrafish somites.

Sun Guangyu G Holley Scott A SA

Molecular & cellular proteomics : MCP 20251008

Integrins are key plasma membrane proteins mediating cell-ECM adhesion and communication and rely on a conformational change for their activation and bidirectional signaling. However, there are few in vivo studies of integrin activation. Here, we identify Integrin α5 (Itgα5) associated proteins in the physiological setting of zebrafish somite morphogenesis. Using label-free mass spectrometry, we compared Itgα5-associated proteins in different integrin activation states. As expected, we found act ...[more]

PMID: 41072919

Similar Datasets

Project description:We sought to characterize cellular heterogeneity in the human cerebral cortex at a molecular level during cortical neurogenesis. We captured single cells and generated sequencing libraries using the C1TM Single-Cell Auto Prep System (Fluidigm), the SMARTer Ultra Low RNA Kit (Clontech), and the Nextera XT DNA Sample Preparation Kit (Illumina). We performed unbiased clustering of the single cells and further examined transcriptional variation among cell groups interpreted as radial glia. Within this population, the major sources of variation related to cell cycle progression and the stem cell niche from which radial glia were captured. We found that outer subventricular zone radial glia (oRG cells) preferentially express genes related to extracellular matrix formation, migration, and stemness, including TNC, PTPRZ1, FAM107A, HOPX, and LIFR and related this transcriptional state to the position, morphology, and cell behaviors previously used to classify the cell type. Our results suggest that oRG cells maintain the subventricular niche through local production of growth factors, potentiation of growth factor signals by extracellular matrix proteins, and activation of self-renewal pathways, thereby contributing to the developmental and evolutionary expansion of the human neocortex. For study version 2, we have updated this data set to include additional primary cells that we infer to represent microglia, endothelial cells, and immature astrocytes, as well as additional cells from the developing neural retina, and from iPS-cell derived cerebral organoids. The genes distinguishing these cell populations may reveal biological processes supporting the diverse functions of these cell types as well as vulnerabilities of specific cell types in human genetic diseases and in viral infections. For study version 3, we have updated the data set to include additional primary cells, including those published in Nowakowski, et al., Science 2017: "Spatiotemporal Gene Expression Trajectories Reveal Developmental Hierarchies of the Human Cortex" (in press)

Dataset Information

Targeted mass spectrum on activation-state-dependent Integrin alpha 5

Publications

Actn4 links inactive Integrin α5 with actin in zebrafish somites.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets