Proteomics

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Development and Validation of a Novel LC-MS/MS Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR)

ABSTRACT: Retinol binding protein 4 (RBP4) is the plasma carrier of retinol that complexes with transthyretin (TTR). A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous absolute quantitation of serum and plasma RBP4 and TTR is needed to advance the understanding of RBP4 and TTR as biomarkers. Surrogate peptides with reproducible, linear LC-MS/MS response were selected for RBP4 and TTR quantitation. Purified proteins were used as quantitation standards and heavy labelled peptides as internal standards. Matrix effects were evaluated for quantitation. The method was validated using pooled human serum and applied to measure inter- and intra-individual variability in RBP4 and TTR concentrations in healthy individuals and in patients with diabetic kidney disease. The assay inter-day variability was <12% and precision within 5%. RBP4 and TTR quantitation correlated with commercially available ELISA assays.

ORGANISM(S): Homo Sapiens

SUBMITTER: Nina Isoherranen  

PROVIDER: PXD061072 | panorama | Fri Sep 26 00:00:00 GMT+01:00 2025

REPOSITORIES: PanoramaPublic

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Development and Validation of a Novel LC-MS/MS-Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR).

Yadav Aprajita S AS   Rubinow Katya B KB   Zelter Alex A   Authement Aurora K AK   Kestenbaum Bryan R BR   Amory John K JK   Isoherranen Nina N  

Journal of proteome research 20250910 10

Retinol binding protein 4 (RBP4), the circulating carrier of retinol, complexes with transthyretin (TTR) and is a potential biomarker of cardiometabolic disease. However, RBP4 quantitation relies on immunoassays and Western blots without retinol and TTR measurement. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous absolute quantitation of circulating RBP4 and TTR is critical to establishing their biomarker potential. Surrogate peptides with reproducible, li  ...[more]

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