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ATI, fructans and predictive assessment of technological quality of Sicilian tetraploid conservation and modern wheat varieties

ABSTRACT: Recently, there has been an increasing interest in wheat landraces and historical varieties mainly cultivated at the beginning of the 19th century, defined as conservation varieties. Most of the Italian tetraploid conservation varieties originate from Sicily, where they are yet boosting the local economy. One of the main reasons for the growing interest in these varieties is the consumers’ perception that they are healthier and safer than their modern counterparts. It is thought that their consumption could ameliorate symptoms of non-celiac wheat sensitivity (NCWS) and irritable bowel syndrome (IBS). Amylase/trypsin-inhibitors (ATIs) and fructans are among the wheat grain components linked to these conditions. In this study we quantified ATI and fructans in five Sicilian conservation varieties (Bidì, Perciasacchi, Russello, Timilia reste bianche, Timilia reste nere) and three modern cultivars (Iride, Orizzonte, Simeto) in a multi-environment trial. Moreover, we calculated the percentage of unextractable polymetric proteins (%UPP) to predict their technological quality. Our findings revealed no significant differences between conservation varieties and modern cultivars for total ATI content, although variations were observed in the composition of individual ATIs. The fructan content was lower in conservation varieties, especially in Bidì and Russello. Overall, %UPP was generally higher in modern cultivars, although Bidì often displayed comparable values. The environmental influence was significant for all analysed traits; however, high heritability was yet observed for all, except for total ATI content. Our results indicate that only minor differences in grain composition exist between tetraploid conservation varieties and modern cultivars.

ORGANISM(S): Triticum Turgidum Subsp. Durum

SUBMITTER: Marco Bonarrigo

PROVIDER: PXD063353 | panorama | Mon Apr 28 00:00:00 BST 2025

REPOSITORIES: PanoramaPublic

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Project description:Wheat (Triticum aestivum ssp. aestivum) contributes to 20% of the human protein supply, delivers essential amino acids and is of fundamental importance for bread and pasta quality. Wheat proteins are also involved in adverse human reactions like celiac disease, wheat allergy and the non-celiac wheat sensitivity (NCWS). Using LC-MS-based LFQ proteomics of aqueous flour extracts we determined 756 proteins across 150 wheat varieties grown in three environments. However, only 303 proteins were stably expressed across all environments in at least one variety underlining the large influence of environmental conditions on the expression of many proteins. Moreover, only 89 proteins were comparably expressed by all 150 varieties, with high coefficients of variation for the other proteins. Heritability (h) ranged from 0-1 with 114 proteins having h² > 0.6. Therefore, the expression of the variable proteins should be amenable to targeted manipulation across the wheat supply chain by varietal choice and breeding. Our study provides a first approach towards a fast and high-throughput methodology for quantifying these proteins which is required to breed wheats with the desired properties. Amylase trypsin inhibitors (ATIs) appear as a potential trigger for NCWS inducing intestinal and extra-intestinal inflammation. Studies on the prevalence and genetic architecture of ATI proteins in wheat are lacking so far. Large differences in the content and composition of 8 ATIs in the different varieties were identified by QconCAT-assisted quantification. The ATI proteins had low coefficients of correlations with quality traits commonly analyzed in wheat breeding. However, heritability was quite low except for ATI 0.28 and ATI CM2. A genome wide association mapping revealed a complex genetic architecture built up on many small but few medium and two major quantitative trait loci (QTL). The latter were on chromosome 3B for ATI 0.19-like and 6B for ATI 0.28 explaining 70.6 and 68.7% of the genetic variance, respectively. Using the wheat reference genome sequence, seven potential candidate genes behind the medium and major QTL were described with only one showing polymorphism based on exome capture analysis. Consequently, wheat breeding could contribute to a reduction of ATI contents in wheat products if incidence of ATI on human health is further confirmed.

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ATI, fructans and predictive assessment of technological quality of Sicilian tetraploid conservation and modern wheat varieties

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