Dataset Information

Absolute quantification of gluten protein groups and their relation to wheat baking quality

ABSTRACT: Wheat gluten proteins are key determinants of baking quality. While untargeted proteomics enable relative quantification, absolute quantities of specific gluten protein groups are not available so far. We developed a targeted liquid chromatography with tandem mass spectrometry (LC-MS/MS) stable isotope dilution assay (SIDA) to quantify eleven individual gluten protein groups based on isotope-labeled internal standards corresponding to the selected marker peptides. The comparison of targeted and untargeted measurements revealed differences in protein composition, likely arising from different MS/MS acquisition strategies and protein assignment. We correlated the absolute protein content with baking quality traits in the Bavarian multiple advanced generation intercross wheat population. None of the individual groups correlated strongly with any baking quality trait. Six groups (α-gliadin 2, γ-gliadin 1, low-molecular-weight glutenin subunit (LMW-GS) 3, and high-molecular-weight glutenin subunits (HMW-GS) 1–4) showed weak to moderate associations (r = 0.32–0.64), mainly with grain protein content, sedimentation value, and wet gluten content. LMW-GS 3 represents the rare i-type containing eight cysteine residues. Loaf volume was only weakly to moderately correlated, primarily with HMW-GS 1 (r = 0.41) and HMW-GS 3 (r = 0.40), supporting the superior effect of Dy10. By contrast, HMW-GS 5 (Dx2, Dx5) showed little effect, consistent with a stronger influence of y-type glutenin subunits. Summing up the protein content across groups increased correlation strengths, yet baking quality remains a complex trait shaped by multiple proteins and non-protein factors.

ORGANISM(S): Triticum Aestivum

SUBMITTER: Christine Kaemper

PROVIDER: PXD072549 | panorama | Thu Mar 05 00:00:00 GMT 2026

REPOSITORIES: PanoramaPublic

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Absolute quantification of gluten protein groups and their relation to wheat baking quality.

Kaemper Christine C Geyer Manuel M Hartl Lorenz L Geisslitz Sabrina S Scherf Katharina Anne KA

Food research international (Ottawa, Ont.) 20251227

Wheat gluten proteins are key determinants of baking quality. While untargeted proteomics enables relative quantification, absolute quantities of specific gluten protein groups are not available so far. We developed a targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) stable isotope dilution assay to quantify eleven individual gluten protein groups based on isotope-labeled internal standards corresponding to selected marker peptides. The comparison of targeted and untargeted meas ...[more]

PMID: 41652760

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Absolute quantification of gluten protein groups and their relation to wheat baking quality

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Absolute quantification of gluten protein groups and their relation to wheat baking quality.

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