Project description:Plasma collected from mice with FIA were pooled, and 0.3 ml was injected intravenously into 6-week-old naïve SJL mice on days 0 and 2. Synovial antigen array profiling of plasma from the arthritic recipient mice demonstrated autoreactive B-cell responses against peptides representing native fibrinogen and citrullinated fibrinogen, and further epitope spreading resulting in additional targeting of fibronectin, collagen type V, cartilage gp39, and clusterin. Custom-spotted protein slides were probed with plasma samples from individual mice. Four slides were probed with plasma derived from naïve mice and four slides were probed with plasma derived from mice injected with FIA plasma.
Project description:This SuperSeries is composed of the following subset Series: GSE24037: Salivary cytokine alterations in HIV infection part 1 GSE24064: Salivary cytokine alterations in HIV infection part 2 Refer to individual Series
Project description:Total RNA was isolated from fresh-frozen tissue of 5 benign and 5 malignant pheochromocytomas. The reference consisted of laser microdissected (LMD) tissue from normal adrenal medullae from 10 different patients. After generating Cy3- and Cy5-fluorescently labeled cDNAs, F-chips containing 11,540 spots were hybridized. Data were analyzed with the IMAGENE 3.0 software.