Project description:The Golgi apparatus from Arabidopsis cell culture (Ler) were enriched by sucrose density centrifugation and purified by Free Flow Electrophoresis. Data from 2 fractions.
Project description:Developing xylem from strong and mild compression wood of Pinus radiata were harvested and Golgi membranes enriched by density centrifugation. Enriched membranes were further purified by free-flow electrophoresis. A total of 3 fractions were collected (from each tissue) and analyzed by tandem mass spectromtery.
Project description:Plasma membranes from 7-day old wild type Arabidopsis seedlings were enriched by two-phase partitioning and further purified by free-flow electrophoresis prior to analysis of fractions by LC-MS/MS. Data from two independent replicates were analyzed, with two fractions analyzed from each replicate.
Project description:Human microbiome contains billions of microorganisms that play important roles in the biological system and different diseases. Due to its complexity, conventional culture-independent technology may underestimate the value of low-abundance bacteria, which calls for a highly efficient method for its enrichment and comprehensive analysis. In this study, we developed a recycling free-flow isoelectric focusing (RFFIEF) method-based electrophoresis method to separate salivary microbiome.
2021-03-10 | PXD024619 |
Project description:Metagenomic analysis of a yeast free dough