Ontology highlight
ABSTRACT:
INSTRUMENT(S): Unknown
ORGANISM(S): Halobacterium Nrc-1 (halobacterium)
SUBMITTER: Y. A. Goo, E. C. Yi, N. S. Baliga, W. A. Tao, M. Pan, R. Aebersold, D. R. Goodlett, L. Hood and W. V. Ng
PROVIDER: PAe000189 | PeptideAtlas | 2006-12-31
REPOSITORIES: PeptideAtlas
Proteomics 20060901 18
In our initial attempt to analyze the human brain proteome, we applied multi-dimensional protein separation and identification techniques using a combination of sample fractionation, 1-D SDS-PAGE, and MS analysis. The complexity of human brain proteome requires multiple fractionation strategies to extend the range and total number of proteins identified. According to the method of Klose (Methods Mol. Biol. 1999, 112, 67), proteins of the temporal lobe of human brain were fractionated into (i) cy ...[more]