Proteomics

Dataset Information

0

Yagsn1_YAGsn2


ABSTRACT: replicate enrichment for soluble proteins; Goo et al., 2003, Mol Cell Proteomics 2(8):509

OTHER RELATED OMICS DATASETS IN: PRJEB2718

INSTRUMENT(S): LTQ

ORGANISM(S): Halobacterium Nrc-1 (halobacterium)

SUBMITTER: Y. A. Goo, E. C. Yi, N. S. Baliga, W. A. Tao, M. Pan, R. Aebersold, D. R. Goodlett, L. Hood and W. V. Ng 

PROVIDER: PAe000249 | PeptideAtlas | 2011-12-31

REPOSITORIES: PeptideAtlas

Dataset's files

Source:
Action DRS
PAe000249_247_search.params.tar.gz Other
PAe000249_247_sequest.params Other
PAe000249_RAW.tar Raw
PAe000249_README Other
PAe000249_Search_Results_247_201104181135.properties Other
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Publications

Deep proteome and transcriptome mapping of a human cancer cell line.

Nagaraj Nagarjuna N   Wisniewski Jacek R JR   Geiger Tamar T   Cox Juergen J   Kircher Martin M   Kelso Janet J   Pääbo Svante S   Mann Matthias M  

Molecular systems biology 20111108


While the number and identity of proteins expressed in a single human cell type is currently unknown, this fundamental question can be addressed by advanced mass spectrometry (MS)-based proteomics. Online liquid chromatography coupled to high-resolution MS and MS/MS yielded 166 420 peptides with unique amino-acid sequence from HeLa cells. These peptides identified 10 255 different human proteins encoded by 9207 human genes, providing a lower limit on the proteome in this cancer cell line. Deep t  ...[more]

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