Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

KidneyAnionEX

ABSTRACT: Mouse Kidney soluable fraction on LCQ

INSTRUMENT(S): LCQ Classic

ORGANISM(S): Mus Musculus

SUBMITTER: Dan Martin

PROVIDER: PAe000269 | PeptideAtlas |

REPOSITORIES: PeptideAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	PAe000269_4935_search.params.tar.gz	Other
	PAe000269_4935_searchdatabase_tandem.fasta	Fasta
	PAe000269_4935_tandem.params	Other
	PAe000269_RAW_201104191005.tar.gz	Other
	PAe000269_README	Other

Items per page:

1 - 5 of 11

Similar Datasets

Multi Omics analysis of fibrotic kidneys in two mouse models (Folic acid (FA) model MS data set)

Project description:Kidney fibrosis represents an urgent unmet clinical need due to the lack of effective therapies and inadequate understanding of the molecular pathogenesis. We have generated a comprehensive and integrated multi-omics data set (proteomics, mRNA and small RNA transcriptomics) of fibrotic kidneys that is searchable through a user-friendly web application. Two commonly used mouse models were utilized: a reversible chemical-induced injury model (folic acid (FA) induced nephropathy) and an irreversible surgically-induced fibrosis model (unilateral ureteral obstruction (UUO)). mRNA and small RNA sequencing as well as 10-plex tandem mass tag (TMT) proteomics were performed with kidney samples from different time points over the course of fibrosis development. The bioinformatics workflow used to process, technically validate, and integrate the single data sets will be described. In summary, we present temporal and integrated multi-omics data from fibrotic mouse kidneys that are accessible through an interrogation tool to provide a searchable transcriptome and proteome for kidney fibrosis researchers.

2019-05-07 | GSE126181 | GEO

Multi Omics analysis of fibrotic kidneys in two mouse models (Unilateral ureter obstruction (UUO) model MS data set)

2019-05-07 | GSE126182 | GEO

BrainWaterSolLCQ

Project description:Mouse brain soluable fraction on LCQ

| PAe000268 | PeptideAtlas

Microarray analysis of mir142 mutant in zebrafish

Project description:The myelomonocyte fraction and whole kidney marrow were sorted from wild-type or mutant zebrafish kidney at 60 days post-fertilization (dpf).

2014-06-24 | E-MTAB-2692 | biostudies-arrayexpress

KidneyAnionLTQ

Project description:Mouse Kidney anion fraction on LTQ

| PAe000270 | PeptideAtlas

Protein Microarrays Discover Angiotensinogen and PRKRIP1 as Novel Targets for Autoantibodies in Chronic Renal Disease

Project description:Biomarkers for early detection of chronic kidney disease are needed, as millions of patients suffer from chronic diseases predisposing them to kidney failure. Protein microarrays may hold utility in the discovery of auto-antibodies in other conditions not commonly considered auto-immune diseases. We hypothesized that proteins are released as a consequence of damage at a cellular level during end-organ damage from renal injury, not otherwise recognized as self-antigens, and an adaptive humoral immune response to these proteins might be detected in the blood, as a non-invasive tracker of this injury. The resultant antibodies (Ab) detected in the blood would serve as effective biomarkers for occult renal injury, enabling earlier clinical detection of chronic kidney disease than currently possible, due to the redundancy of the serum creatinine as a biomarker for early kidney injury. To screen for novel autoantibodies in chronic kidney disease, 24 protein microarrays were used to compare serum Ab from patients with chronic kidney disease against matched controls. From a panel of 38 antigens with increased Ab binding, 4 were validated in 71 individuals, with (n=50) and without (n=21) renal insufficiency. Significant elevations in the titer of novel auto-Ab were noted against Angiotensinogen (AGT) and PRKRIP1 in renal insufficiency. Current validation is underway to evaluate if these auto-Ab can provide means to follow the evolution of chronic kidney disease in patients with early stages of renal insufficiency, and if these rising titers of these auto-Ab correlate with the rate of progression of chronic kidney disease.

2010-08-13 | GSE23609 | GEO

Small non-coding RNA expression in developing mouse nephron progenitors

Project description:The overall goal of this study is to determine the small non-coding RNA expression profile in developing mouse nephron progenitors and whole kidney. Using a limited digestion and negative selection approach, an enriched fraction of nephron progenitors were isolated from E15.5 whole kidney samples followed by small RNA-Sequencing (sRNA-Seq). A total of 3 biological replicates of mouse nephron progenitors and whole kidney samples were used for the sRNA-Seq. The NEBNext Multiplex Small RNA Library Prep Kit allowed us to prepare sequencing libraries from as little as 100ng total RNA. Multiplex sequencing was performed using the Illumina NextSeq 550 system with 50bp single reads, resulting in approximately 18 million reads per sample. Reads were aligned to the mm10 genome using Bowtie2, and the miRDeep2 software package was used to identify and quantify known and novel micro RNA (miRNA) within our sRNA-seq libraries. Differential expression analysis of miRNA expression between nephron progenitor and whole kidney samples identified 162 differentially expressed miRNAs (padj <= 0.05).

2018-08-21 | GSE111729 | GEO

Injured Mouse Kidney

Project description:Injured Mouse Kidney

| PRJNA807744 | ENA

BrainOctylSolLCQ

Project description:Mouse Brain Octyl soluable fraction on LCQ

| PAe000273 | PeptideAtlas

The glomerular circadian clock temporally regulates basement membrane dynamics and podocyte glucocorticoid response

Project description:Kidneys were collected from wildtype mice over the course of two circadian cycles (every 4 hours for 48 hours), snap frozen, and later prepared for circadian proteomic analysis The purpose of this study was to investigate the correlation between rhythmic matrix gene expression and matrix protein abundance. To focus our analysis on the kidney matrix, we generated a matrix-enriched fraction. Samples were prepared for label-free mass spectrometry-based proteomics to define rhythmic changes in the protein composition of mouse kidneys.

2026-02-02 | PXD054668 | Pride