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Heart_Micro_UT

ABSTRACT: Heart Microsomal Fraction - UT

INSTRUMENT(S): LCQ DECA XP

ORGANISM(S): Mus Musculus

SUBMITTER: Andrew Emili

PROVIDER: PAe000354 | PeptideAtlas |

REPOSITORIES: PeptideAtlas

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Dataset's files

Source:

			Action	DRS
	PAe000354_4980_search.params.tar.gz	Other
	PAe000354_4980_searchdatabase_tandem.fasta	Fasta
	PAe000354_4980_tandem.params	Other
	PAe000354_RAW_201104190509.tar.gz	Other
	PAe000354_README	Other

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Identification of the UT-A1 Urea Channel Interactome

Project description:The urea channel Slc14a2 (or UT-A1) mediates vasopressin-regulated urea transport across the inner medullary collecting duct (IMCD). Previously, UT-A1 was found to present in a high molecular weight complex, suggesting UT-A1 is involved in certain protein-protein interactions. The present study sought to identify the proteins that interact with UT-A1 in this complex for a better understanding of how UT-A1 is regulated. Rat IMCD suspensions were treated with or without V2 receptor agonist, dDAVP, followed by in-cell crosslinking using BSOCOES and detergent solubilization. Immunoprecipitation using Dynabeads coated with UT-A1 specific antibody successfully pulled down the UT-A1 proteins. In-gel digestion protocol was carried out to prepare samples for liquid chromatographic mass spectrometry analysis of tryptic peptides using a Velos-Orbitrap mass spectrometer. The peptides passing stringent spectral quality thresholds were quantified (label-free) to identify those with (UTA-1 antibody/preimmune IgG) >4. A total of 128 UT-A1 interacting proteins were identified. Gene Ontology analysis maps the distribution of these proteins throughout major cell compartments: endoplasmic reticulum, Golgi, endosomes, cytosol and plasma membrane. Among them are four protein kinases (Cdc42bpb, Phkb, Camk2d, Mtor) that play roles in vasopressin-regulated phosphorylation of UT-A1. Non-label quantification was also performed to determine the stoichiometry of UT-A3 with UT-A1, the result does not support an oligomeric complex formation of UT-A1/A3. In conclusion, we have provided a refined list of UT-A1 binding proteins which can be useful for further analysis of the vasopressin signaling pathway in regulation of UT-A1 in IMCD.

2019-11-08 | PXD003496 | Pride

tableNONMMUT140591.1 may serve as a ceRNA to regulate Gata5 in UT-B knockout-induced cardiac conduction block

Project description:Objective: We intended to explore the potential molecular mechanisms underlying cardiac conduction block inducted by UT-B deletion at the transcriptome level. Methods: The heart tissues were harvested from UT-B null mice and age-matched wild-type mice for lncRNA sequencing analysis. Based on the sequencing data, the differentially expressed mRNAs (DEMs) and lncRNAs (DELs) between UT-B knockout and control groups were identified, followed by function analysis and mRNA-lncRNA co-expression analysis. The miRNAs were predicted, and then the competing endogenous RNA (ceRNA) network was constructed. Results: UT-B deletion results in the aberrant expression of 588 lncRNAs and 194 mRNAs. These DEMs were significantly enriched in inflammation-related pathway. A lncRNA-mRNA co-expression network and a ceRNA network was constructed on the basis of the DEMs and DELs. C7 (complement C7)-NONMMUT137216.1 co-expression pair had the highest correlation coefficient in the co-expression network. NONMMUT140591.1 had the highest degree in ceRNA network and involved in ceRNA of NONMMUT140591.1-mmu-miR-298-5p-Gata5 (GATA binding protein 5). Conclusion: UT-B deletion may promote cardiac conduction block via inflammatory process. The ceRNA NONMMUT140591.1-mmu-miR-298-5p-Gata5 may be a potential molecular mechanism of UT-B knockout-induced cardiac conduction block.

2021-03-12 | GSE168717 | GEO

A_niger_microsome - Proteomic analysis of the secretory response of Aspergillus niger to D-maltose and D-xylose

Project description:Shotgun proteomics of microsomal enriched fraction 2 h after addition 0f 10mM xylose to sorbitol grown mycelium

2012-05-17 | PRD000334 | Pride

Placenta_Micro_UT

Project description:Placenta Microsomal Fraction - UT

| PAe000381 | PeptideAtlas

Liver_Micro_UT

Project description:Liver Microsomal Fraction - UT

| PAe000376 | PeptideAtlas

Lung_Micro_UT

Project description:Lung Microsomal Fraction - UT

| PAe000363 | PeptideAtlas

Brain_Micro_UT

Project description:Brain Microsomal Fraction - UT

| PAe000370 | PeptideAtlas

Kidney_Micro_UT

Project description:Kidney Microsomal Fraction - UT

| PAe000368 | PeptideAtlas

Autoantibodies secreted by TLR7-stimulated B cell subsets from mice with primary Sjogren's syndrome

Project description:Spleens (n = 2-3) were harvested from mice with primary Sjogren's syndrome (NOD.B10) and cells were sort-purified. Follicular B cells (FO) (6 million), Marginal zone B cells (4.5 million) and age-associated B cells (500,000) were sorted. Cells were cultured in 200 uL of complete RPMI media containing 62.5 ng/mL of imiquimod, a TLR7 agonist. Cells were cultured for 6 days, and the supernatants were harvested and stored at -20 C. Samples were shipped to UT Southwestern for autoantigen array analysis.

2023-10-24 | GSE236255 | GEO

Heart_Cyto_UT

Project description:Heart Cytoplasmic Fraction - UT

| PAe000379 | PeptideAtlas