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Brain_Mito_UT

ABSTRACT: Brain Mitochondrial Fraction - UT

INSTRUMENT(S): LCQ DECA XP

ORGANISM(S): Mus Musculus

SUBMITTER: Andrew Emili

PROVIDER: PAe000359 | PeptideAtlas |

REPOSITORIES: PeptideAtlas

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Dataset's files

Source:

			Action	DRS
	PAe000359_4942_search.params.tar.gz	Other
	PAe000359_4942_searchdatabase_tandem.fasta	Fasta
	PAe000359_4942_tandem.params	Other
	PAe000359_RAW_201104190323.tar.gz	Other
	PAe000359_README	Other

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Identification of the UT-A1 Urea Channel Interactome

Project description:The urea channel Slc14a2 (or UT-A1) mediates vasopressin-regulated urea transport across the inner medullary collecting duct (IMCD). Previously, UT-A1 was found to present in a high molecular weight complex, suggesting UT-A1 is involved in certain protein-protein interactions. The present study sought to identify the proteins that interact with UT-A1 in this complex for a better understanding of how UT-A1 is regulated. Rat IMCD suspensions were treated with or without V2 receptor agonist, dDAVP, followed by in-cell crosslinking using BSOCOES and detergent solubilization. Immunoprecipitation using Dynabeads coated with UT-A1 specific antibody successfully pulled down the UT-A1 proteins. In-gel digestion protocol was carried out to prepare samples for liquid chromatographic mass spectrometry analysis of tryptic peptides using a Velos-Orbitrap mass spectrometer. The peptides passing stringent spectral quality thresholds were quantified (label-free) to identify those with (UTA-1 antibody/preimmune IgG) >4. A total of 128 UT-A1 interacting proteins were identified. Gene Ontology analysis maps the distribution of these proteins throughout major cell compartments: endoplasmic reticulum, Golgi, endosomes, cytosol and plasma membrane. Among them are four protein kinases (Cdc42bpb, Phkb, Camk2d, Mtor) that play roles in vasopressin-regulated phosphorylation of UT-A1. Non-label quantification was also performed to determine the stoichiometry of UT-A3 with UT-A1, the result does not support an oligomeric complex formation of UT-A1/A3. In conclusion, we have provided a refined list of UT-A1 binding proteins which can be useful for further analysis of the vasopressin signaling pathway in regulation of UT-A1 in IMCD.

2019-11-08 | PXD003496 | Pride

Kidney_Mito_UT

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Project description:Lung Mitochondrial Fraction - UT

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Project description:Heart Mitochondrial Fraction - UT

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Liver_Mito_UT

Project description:Liver Mitochondrial Fraction - UT

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Label free quantitative analysis of brain mitochondrial fraction tumour vs non-tumour

Project description:Homo sapiens; non-tumourous; brain mitochondrial fraction

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Autoantibodies secreted by TLR7-stimulated B cell subsets from mice with primary Sjogren's syndrome

Project description:Spleens (n = 2-3) were harvested from mice with primary Sjogren's syndrome (NOD.B10) and cells were sort-purified. Follicular B cells (FO) (6 million), Marginal zone B cells (4.5 million) and age-associated B cells (500,000) were sorted. Cells were cultured in 200 uL of complete RPMI media containing 62.5 ng/mL of imiquimod, a TLR7 agonist. Cells were cultured for 6 days, and the supernatants were harvested and stored at -20 C. Samples were shipped to UT Southwestern for autoantigen array analysis.

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Brain_Micro_UT

Project description:Brain Microsomal Fraction - UT

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Brain_Cyto_UT

Project description:Brain Cytoplasmic Fraction - UT

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