Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hela_02_trypsin

ABSTRACT: Cancer Cell line - Hela 02 trypsin

OTHER RELATED OMICS DATASETS IN: PRJEB2718

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Nagaraj N, Wisniewski JR, Geiger T, Cox J, Kircher M, Kelso J, P��bo S, Mann M

PROVIDER: PAe003653 | PeptideAtlas | 2011-12-31

REPOSITORIES: PeptideAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	PAe003653_7166_tandem.params	Other
	PAe003653_README	Other
	PAe003653_Search_Results_7166_201211210132.properties	Other
	PAe003653_Search_Results_7166_201211210132.tar.gz	Other
	PAe003653_mzML_201211202054.properties	Other

Items per page:

1 - 5 of 9

Publications

Deep proteome and transcriptome mapping of a human cancer cell line.

Nagaraj Nagarjuna N Wisniewski Jacek R JR Geiger Tamar T Cox Juergen J Kircher Martin M Kelso Janet J Pääbo Svante S Mann Matthias M

Molecular systems biology 20111108

While the number and identity of proteins expressed in a single human cell type is currently unknown, this fundamental question can be addressed by advanced mass spectrometry (MS)-based proteomics. Online liquid chromatography coupled to high-resolution MS and MS/MS yielded 166 420 peptides with unique amino-acid sequence from HeLa cells. These peptides identified 10 255 different human proteins encoded by 9207 human genes, providing a lower limit on the proteome in this cancer cell line. Deep t ...[more]

PMID: 22068331

Similar Datasets

Hela_02_GluC

Project description:Cancer Cell line - Hela 02 Gluc

2011-12-31 | PAe003654 | PeptideAtlas

Hela_02_Lysc

Project description:Cancer Cell line - Hela 02 Lysc

2011-12-31 | PAe003658 | PeptideAtlas

C1R.A*01:01, C1R.A*02:01, C1R.A*24:02 MHC class I immunopeptidome LC-MSMS

Project description:Characterisation of peptide ligands of Major histocompatibility class (MHC) I isolated by immunoaffinity purification from the C1R (Class I reduced) B-lymphoblastoid cell line, transfected with the MHC class I allele HLA-A*01:01, or HLA-A*02:01, HLA-A*24:02. In addition, public mass spectrometry (MS) datasets of HLA-I and HLA-II immunopeptidome derived from patients’ samples, PBMC or cell lines, and shotgun proteomics from trypsin/elastase digestion were analysed.

2020-07-27 | PXD014754 | Pride

The effect of trypsin-2 on human tongue squamous cell carcinoma cell line gene expression

Project description:The level of trypsin-2 has been shown to correlate with the malignancy and metastatic potential of many cancer. We used microarrays to detail how tryspin-2 overexpression changes the gene profile of human tongue squamous cell carcinoma cell line (HSC-3). We compared trypsin-2 overexpressing HSC-3 line to HSC-3 which was transfected with control vector and because of that did not expressed trypsin-2 at all.

2012-10-02 | E-GEOD-32056 | biostudies-arrayexpress

Hela_01_trypsin

Project description:Cancer Cell line - Hela 01 trypsin

2011-12-31 | PAe003661 | PeptideAtlas

Homo sapiens

Project description:Cell division cycle in a human cancer cell line (HeLa)

| PRJNA93325 | ENA

The effect of trypsin-2 on human tongue squamous cell carcinoma cell line gene expression

2012-10-02 | GSE32056 | GEO

Project description:Human HeLa cell line transcriptome

| PRJNA791826 | ENA

Enhanced anti-cancer effects of conditioned medium from hypoxic human adult dermal fibroblasts on cervical cancer cells [dataset 2]

Project description:Hypoxia regulates fibroblast function by changing intracellular signaling and secretion factors, which influences the states of nearby cells. In this work, we investigated the effects of conditioned medium (CM) from human adult dermal fibroblasts (HDFs) cultured in normoxic and hypoxic conditions on cervical cancer (HeLa) cells. The HeLa cells showed decreased cell viability, in-creased apoptosis, and cell cycle arrest in response to CM from hypoxic-cultured HDFs (H-CM) compared with CM from normoxic-cultured HDFs (N-CM). Among the proteins up-regulated (> 2-fold) in H-CM compared with N-CM, LTBR decreased the viability of HeLa cells. Among the intracellular proteins down-regulated (> 2-fold) in HeLa cells treated with H-CM compared with N-CM, the most enriched biological process GO term and KEGG pathway were protein deubiq-uitination and hsa05166:HTLV-I infection, respectively. In the protein–protein interaction net-work of intracellular proteins with altered expression (> 2-fold), 1 up-regulated (TNF) and 8 down-regulated (ESR1, MCL1, TBP, CD19, LCK, PCNA, CHEK1, and POLA1) hub proteins were defined. Among the down-regulated hub proteins, the most enriched biological process GO term and KEGG pathway were leading strand elongation and hsa05166:HTLV-I infection, respectively. This study reveals that H-CM had enhanced anti-cancer effects on cervical cancer cells compared with N-CM, and it induced intracellular signaling patterns related to those enhanced anti-cancer effects.

2022-03-04 | GSE185979 | GEO

Enhanced anti-cancer effects of conditioned medium from hypoxic human adult dermal fibroblasts on cervical cancer cells [dataset 1]

2022-03-04 | GSE185978 | GEO