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Hs_GlobalSubcellular_SILAC

ABSTRACT: Characterisation of PRR11

REANALYSIS of: PXD000262

INSTRUMENT(S): QExactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Larance M, Ahmad Y, Kirkwood KJ, Ly T, Lamond AI

PROVIDER: PAe005033 | PeptideAtlas | 2013-12-31

REPOSITORIES: PeptideAtlas

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Global subcellular characterization of protein degradation using quantitative proteomics.

Larance Mark M Ahmad Yasmeen Y Kirkwood Kathryn J KJ Ly Tony T Lamond Angus I AI

Molecular & cellular proteomics : MCP 20121212 3

Protein degradation provides an important regulatory mechanism used to control cell cycle progression and many other cellular pathways. To comprehensively analyze the spatial control of protein degradation in U2OS osteosarcoma cells, we have combined drug treatment and SILAC-based quantitative mass spectrometry with subcellular and protein fractionation. The resulting data set analyzed more than 74,000 peptides, corresponding to ~5000 proteins, from nuclear, cytosolic, membrane, and cytoskeletal ...[more]

PMID: 23242552

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