Proteomics

Dataset Information

0

Hs_Phospho_Jurkat_CID

ABSTRACT: Ti4+-IMAC phosphopeptide enrichment, CID, Jurkat T-cells

REANALYSIS of: PXD000293

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Albert Heck 

PROVIDER: PAe005133 | PeptideAtlas | 2014-12-31

REPOSITORIES: PeptideAtlas

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Publications

Single-step enrichment by Ti4+-IMAC and label-free quantitation enables in-depth monitoring of phosphorylation dynamics with high reproducibility and temporal resolution.

de Graaf Erik L EL   Giansanti Piero P   Altelaar A F Maarten AF   Heck Albert J R AJ  

Molecular & cellular proteomics : MCP 20140521 9

Quantitative phosphoproteomics workflows traditionally involve additional sample labeling and fractionation steps for accurate and in-depth analysis. Here we report a high-throughput, straightforward, and comprehensive label-free phosphoproteomics approach using the highly selective, reproducible, and sensitive Ti(4+)-IMAC phosphopeptide enrichment method. We demonstrate the applicability of this approach by monitoring the phosphoproteome dynamics of Jurkat T cells stimulated by prostaglandin E2  ...[more]