Proteomics

Dataset Information

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Hs_HESC_Phospho_Silac_PMA_2

ABSTRACT: hESCs cultured treated with PMA for 0min, 1hr or 24hr

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Matthias Mann 

PROVIDER: PAe005235 | PeptideAtlas | 2011-12-31

REPOSITORIES: PeptideAtlas

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PAe005235_10209_tandem.params Other
PAe005235_README Other
PAe005235_Search_Results_10209_201512312300.properties Other
PAe005235_Search_Results_10209_201512312300.tar.gz Other
PAe005235_mzXML_201512312245.properties Other
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Publications

System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

Rigbolt Kristoffer T G KT   Prokhorova Tatyana A TA   Akimov Vyacheslav V   Henningsen Jeanette J   Johansen Pia T PT   Kratchmarova Irina I   Kassem Moustapha M   Mann Matthias M   Olsen Jesper V JV   Blagoev Blagoy B  

Science signaling 20110315 164

To elucidate cellular events underlying the pluripotency of human embryonic stem cells (hESCs), we performed parallel quantitative proteomic and phosphoproteomic analyses of hESCs during differentiation initiated by a diacylglycerol analog or transfer to media that had not been conditioned by feeder cells. We profiled 6521 proteins and 23,522 phosphorylation sites, of which almost 50% displayed dynamic changes in phosphorylation status during 24 hours of differentiation. These data are a resourc  ...[more]

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