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Hs_HESC_Phospho_Silac_NCM_1

ABSTRACT: hESCs cultured treated with nonconditioned medium (NCM) for 0min, 30min or 6hr.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Matthias Mann

PROVIDER: PAe005238 | PeptideAtlas | 2011-12-31

REPOSITORIES: PeptideAtlas

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			Action	DRS
	PAe005238_10219_tandem.params	Other
	PAe005238_README	Other
	PAe005238_Search_Results_10219_201512312141.properties	Other
	PAe005238_Search_Results_10219_201512312141.tar.gz	Other
	PAe005238_mzXML_201512312125.properties	Other

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1 - 5 of 8

Publications

System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

Rigbolt Kristoffer T G KT Prokhorova Tatyana A TA Akimov Vyacheslav V Henningsen Jeanette J Johansen Pia T PT Kratchmarova Irina I Kassem Moustapha M Mann Matthias M Olsen Jesper V JV Blagoev Blagoy B

Science signaling 20110315 164

To elucidate cellular events underlying the pluripotency of human embryonic stem cells (hESCs), we performed parallel quantitative proteomic and phosphoproteomic analyses of hESCs during differentiation initiated by a diacylglycerol analog or transfer to media that had not been conditioned by feeder cells. We profiled 6521 proteins and 23,522 phosphorylation sites, of which almost 50% displayed dynamic changes in phosphorylation status during 24 hours of differentiation. These data are a resourc ...[more]

PMID: 21406692

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Achieving Stable X-chromosome Inactivation in Human Embryonic Stem Cells

Project description:X-inactivation is a paradigm of epigenetic transcriptional regulation. Human embryonic stem cells (hESCs) that harbor an inactivated X-chromosome often undergo erosion of X-inactivation upon prolonged culture. Here, we investigate the sources of X-inactivation instability by deriving new primed pluripotent hESC lines. We find that the composition of culture media dramatically influenced the expression of XIST lncRNA, a key regulator of X-inactivation. hESCs cultured in a defined medium stably maintained XIST RNA coating, whereas hESCs cultured in the widely-used mTeSR1 medium lost XIST RNA expression. We pinpointed lithium chloride in mTeSR1 as a cause of XIST RNA loss. The addition of LiCl or inhibitors of glycogen synthase kinase 3 (GSK-3) proteins, which are inhibited by lithium, to the defined hESC culture medium impeded XIST expression. Together, these data may reconcile the observed variations in X-inactivation in hESCs and inform the culture of pluripotent stem cells for disease modeling and regenerative medicine.

2022-03-11 | GSE157809 | GEO

ZIC2 and ZIC3 promote SWI/SNF recruitment to safeguard progression towards human primed pluripotency [ATAC-Seq]

Project description:ZIC2 and ZIC3 are essential for acquisition and maintenance of primed pluripotency. In this study, we conducted ATAC-seq in single and compound knockout of ZIC2, ZIC3 and ZIC5 primed hESCs, naïve hESCs lacking ZIC3 that inducibly express ZIC2, naive hESCs that inducible express ZIC2 or ZIC3, ZIC3-depleted primed hESCs that were cultured in 5iLAF medium for six days and undergoing reversion to a naive state, naive hESCs expressing ectopic ZIC2 in the presence of a BRG1 PROTAC.

2024-08-20 | GSE270948 | GEO

ZIC2 and ZIC3 promote SWI/SNF recruitment to safeguard progression towards human primed pluripotency [RNA-seq]

Project description:ZIC2 and ZIC3 are essential for acquisition and maintenance of primed pluripotency. In this study, we conducted next generation sequencing of polyA-enriched bulk RNA in single and compound knockouts of ZIC2, ZIC3 and ZIC5 primed hESCs, naïve hESCs lacking ZIC3 that inducibly express ZIC2, and primed hESCs that were cultured in 5iLAF medium for six days and undergoing reversion to a naive state.

2024-08-20 | GSE270786 | GEO

Hs_HESC_Phospho_Silac_NCM_2r

Project description:hESCs cultured treated with nonconditioned medium (NCM) for 0min, 1hr or 24hr.

2011-12-31 | PAe005298 | PeptideAtlas

Hs_HESC_Phospho_Silac_NCM_1r

Project description:hESCs cultured treated with nonconditioned medium (NCM) for 0min, 30min or 6hr.

2011-12-31 | PAe005244 | PeptideAtlas

Hs_HESC_Phospho_Silac_NCM_2

Project description:hESCs cultured treated with nonconditioned medium (NCM) for 0min, 1hr or 24hr.

2011-12-31 | PAe005255 | PeptideAtlas

Capturing totipotency in human cells through spliceosomal repression [RNA-Seq]

Project description:Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cellb blastomeres Poly(A) RNA-seq is performed on hESCs cultured in MYCP medium

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Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) revealed genomic chromatin accessibilities change induced by loss of ARID1A in differentiated (day 4) H9 hESCs

Project description:We reported loss of ARID1A promoted neurogenesis and inhibited cardiogenesis. Under microscopy, we observed that spontaneously differentiated cells were induced in ARID1A KO H9 hESCs cultured in mTesR medium. We did not know what cells types were. Here ATAC-seq were used to investigate chromatin accessibilities change in differentiated (day 4) WT H9 hESCs and ARID1A KO hESC cells.

2020-06-19 | GSE139329 | GEO

Single cell RNA-seq revealed different cell types induced by loss of ARID1A in undifferentiated and differentiation (day 10) H9 hESCs.

Project description:We reported loss of ARID1A promoted neurogenesis and inhibited cardiogenesis. Under microscopy, we observed that spontaneously differentiated cells were induced in ARID1A KO H9 hESCs cultured in mTesR medium. After cardiac differentiation for 10 days, we also observed the cell types were totally different between WT and ARID1A KO cells. We did not know what cells types were. Here scRNA-seq were used to identify the cell types in WT H9 hESCs and ARID1A KO H9 hESCs.

2020-06-19 | GSE139342 | GEO

Transcription profiling of Thermus thermophilus HB8 wild-type strain grown on a rich medium

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Experiment Overall Design: wild-type T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.3 ml) were inoculated into 0.15 liter of the same medium and then cultivated at 70oC. At the Abs600=0.8 (0min), cold medium was added and the temperature were shifted down to 45oC. Cells were collected at 0min and 30min after temperature shift, and then crude RNA was extracted. In order to compare mRNA expression in thewild-type cell between 45oC and 70oC, the expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2009-01-09 | E-GEOD-12437 | biostudies-arrayexpress