Dataset Information
Hs_Phospho_AspN_ETD
Ontology highlight
ABSTRACT:
REANALYSIS of: PXD001428
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Others
SUBMITTER: Piero Giansanti
PROVIDER: PAe005250 | PeptideAtlas | 2015-12-31
REPOSITORIES: PeptideAtlas
Publications
An Augmented Multiple-Protease-Based Human Phosphopeptide Atlas.
Cell reports 20150611 11
Although mass-spectrometry-based screens enable thousands of protein phosphorylation sites to be monitored simultaneously, they often do not cover important regulatory sites. Here, we hypothesized that this is due to the fact that nearly all large-scale phosphoproteome studies are initiated by trypsin digestion. We tested this hypothesis using multiple proteases for protein digestion prior to Ti(4+)-IMAC-based enrichment. This approach increases the size of the detectable phosphoproteome substan ...[more]
