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Hs_Hela_CellCycle_Phospho

ABSTRACT: HeLa S3 cells, SILAC labeled

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Matthias Mann

PROVIDER: PAe005252 | PeptideAtlas | 2010-12-31

REPOSITORIES: PeptideAtlas

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			Action	DRS
	PAe005252_10244_tandem.params	Other
	PAe005252_README	Other
	PAe005252_Search_Results_10244_201512311525.properties	Other
	PAe005252_Search_Results_10244_201512311525.tar.gz	Other
	PAe005252_mzXML_201512311433.properties	Other

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Publications

Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

Olsen Jesper V JV Vermeulen Michiel M Santamaria Anna A Kumar Chanchal C Miller Martin L ML Jensen Lars J LJ Gnad Florian F Cox Jürgen J Jensen Thomas S TS Nigg Erich A EA Brunak Søren S Mann Matthias M

Science signaling 20100112 104

Eukaryotic cells replicate by a complex series of evolutionarily conserved events that are tightly regulated at defined stages of the cell division cycle. Progression through this cycle involves a large number of dedicated protein complexes and signaling pathways, and deregulation of this process is implicated in tumorigenesis. We applied high-resolution mass spectrometry-based proteomics to investigate the proteome and phosphoproteome of the human cell cycle on a global scale and quantified 602 ...[more]

PMID: 20068231

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Project description:Treatment with the histone deacetylase inhibitor trichostatin a (TSA) changes the radial positioning of the CFTR gene in HeLa S3 cells. The gene relocates from the nuclear periphery to the nuclear interior. In Calu-3 cells the gene is located in the nuclear interior. To identifiy potential regulatory elements for the positioning of CFTR, the histone h3 and h4 acetylation patterns of untreated and TSA-treated HeLa S3 and untreated Calu-3 cells were determined by ChIP-chip. A CTCF site close to the CFTR promoter displayed consistent histone H3 hyperacetylation in TSA treated HeLa S3 cells and Calu-3 cells. Four Agilent 4x 44K DNA-chips (DNA-Chips: REP_1 - REP_4). HeLa untreated and TSA treated: H3ac/H3pan (Cy5/Cy3): hybridized together on four sectors - 1x regular 3x dye swap; H4ac: 3 sectors, respective H3pan reference on different sectors on the same DNA-chips (labeled with the identical fluorophore). Calu-3: H3ac/h3pan: one sector each on the same DNA-chip (labeled with the identical fluorophore) H4ac/H3pan: two sectors each on the same DNA-chips (two each on two DNA-Chips, labeled with the identical fluorophore)

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