Dataset Information
Hs_Plk1wt_Phospho_Silac
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Others
SUBMITTER: Henrik Daub
PROVIDER: PAe005299 | PeptideAtlas | 2012-12-31
REPOSITORIES: PeptideAtlas
Dataset's files
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Publications
Combination of chemical genetics and phosphoproteomics for kinase signaling analysis enables confident identification of cellular downstream targets.
Molecular & cellular proteomics : MCP 20111222 4
Delineation of phosphorylation-based signaling networks requires reliable data about the underlying cellular kinase-substrate interactions. We report a chemical genetics and quantitative phosphoproteomics approach that encompasses cellular kinase activation in combination with comparative replicate mass spectrometry analyses of cells expressing either inhibitor-sensitive or resistant kinase variant. We applied this workflow to Plk1 (Polo-like kinase 1) in mitotic cells and induced cellular Plk1 ...[more]
