Project description:Second-harmonic generation microendoscopy is a minimally invasive technique to image sarcomeres and measure their lengths in humans, but motion artifact and low signal have limited the use of this novel technique.We discovered that an excitation wavelength of 960 nm maximized image signal; this enabled an image acquisition rate of 3 frames/s, which decreased motion artifact. We then used microendoscopy to measure sarcomere lengths in the human extensor carpi radialis brevis with the wrist at 45° extension and 45° flexion in 7 subjects. We also measured the variability in sarcomere lengths within single fibers.Average sarcomere lengths in 45° extension were 2.93±0.29 ?m (±SD) and increased to 3.58±0.19 ?m in 45° flexion. Within single fibers the standard deviation of sarcomere lengths in series was 0.20 ?m.Microendoscopy can be used to measure sarcomere lengths at different body postures. Lengths of sarcomeres in series within a fiber vary substantially.
Project description:Lateral epicondylitis, or tennis elbow, has been attributed to the degeneration of the extensor carpi radialis brevis tendon, with surgery reserved for recalcitrant cases. Surgical intervention of lateral epicondylitis includes release alone or release with repair. Outcomes of open surgical repair have been reported with better preservation of the grip strength than release alone. We describe a technique for the release and repair of the extensor carpi radialis brevis tendon using an arthroscopic approach, which could be used to repair the extensor carpi radialis brevis with minimal invasion and to address the concomitant intra-articular pathology.
Project description:Recalcitrant elbow lateral epicondylitis presents a difficult challenge for both the patient and the doctor. Surgical treatment consists of open resection and debridement of the extensor carpi radialis brevis muscle. However, advances in arthroscopic surgery provide for a more elegant method. There are several comparative studies between arthroscopic and open treatment that have shown equivalent outcomes. There is no consensus on which treatment is better and no defined indications of when to choose one over the other. This article presents our technique for arthroscopic extensor carpi radialis brevis muscle resection.
Project description:Purpose To evaluate outcomes of patients who underwent small-bore needle arthroscopic extensor carpi radialis brevis (ECRB) release for the management of recalcitrant lateral epicondylitis. Methods Patients who underwent elbow evaluation and ECRB release using a small-bore needle arthroscopy system were included in this study Thirteen patients were included. Quick Disabilities of the Arm Shoulder and Hand and Single Assessment Numerical Evaluation scores, as well as overall satisfaction scores, were collected. A 2-tailed paired t-test was conducted to assess the statistical significance of the differences observed between preoperative and 1-year postoperative scores with significance set at P < .05. Results There was a statistically significant improvement in both outcome measures (P < .001) and a 92.3% satisfaction rate with no significant complications at a minimum 1-year follow-up. Conclusions Patients with recalcitrant lateral epicondylitis treated with ECRB release using needle arthroscopy demonstrated significantly improved Quick Disabilities of the Arm Shoulder and Hand and Single Assessment Numerical Evaluation scores postoperatively, without complications. Level of Evidence IV, retrospective case series.
Project description:IntroductionThere is no consensus on treatment of recalcitrant lateral epicondylitis (RLE). This is a prospective, non-randomized, interventional study comparing pain scores and functional outcomes between arthroscopic extensor carpi radialis brevis release and continued intensive conservative treatment.Materials and methodsThe study compared two groups: Group 1, consisting of 25 patients undergoing continued conservative treatment for 24 months, and Group 2, consisting of 25 patients undergoing arthroscopic extensor carpi radialis brevis release with decortication (ARD). VAS (Visual Analogue Scale) score for lateral elbow pain at rest and after routine daily activities were compared at 6 weeks, 24 weeks, 12 months and 24 months. Functional outcomes were compared with grip strength, and patients reported functional outcome scores, pre-intervention and 24 months post-intervention.ResultsThere was a significant improvement in VAS scores for pain, functional outcome scores, and grip strength in both the groups post-intervention (P < 0.05). VAS scores for pain at rest in both the groups were significantly better after the interventions, at all follow-up durations (P < 0.001). VAS scores for pain after routine daily activities were significantly better in group 2 at 24 weeks (P = 0.002) and afterward (P < 0.001). Group 2 had significantly better functional outcome scores at 24 months (P < 0.001) though the difference in grip strength was not statistically significant (P = 0.121).ConclusionThe present study shows favourable functional outcomes and pain scores of ARD compared to continued intensive conservative treatment for RLE.Level of studyII, Non-randomized comparative study.Supplementary informationThe online version contains supplementary material available at 10.1007/s43465-022-00649-w.
Project description:The presence of anti-microbial phenolic compounds, such as the model compound ferulic acid, in biomass hydrolysates poses significant challenges to the widespread use of biomass in conjunction with whole cell biocatalysis or fermentation. Currently, these inhibitory compounds must be removed through additional downstream processing to create feedstock suitable for most industrially important microbial strains. This study explores the high ferulic acid tolerance in Lactobacillus brevis (L. brevis), a lactic acid bacteria often found in fermentation processes, by global transcriptional response analysis. The transcriptional profile of L. brevis under ferulic acid stress reveals that the presence of ferulic acid primarily triggers the expression of membrane proteins to counteract ferulic acid induced changes in membrane fluidity and ion leakage, in the midst of a generalized stress response. Several promising routes for understanding phenolic acid tolerance have been identified based upon these findings. These insights may be used to guide further engineering of model industrial organisms to better tolerate phenolic compounds in processed biomass.