Dataset Information
Differential COFRADIC N-terminal proteomic analysis of Fas-induced apoptotic protein cleavage in Jurkat cells
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model, Waters instrument model
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Jurkat Cell
SUBMITTER: 
Lennart Martens
PROVIDER: PRD000008 | Pride | 2005-12-22
REPOSITORIES: Pride
Publications
Caspase-specific and nonspecific in vivo protein processing during Fas-induced apoptosis.
Nature methods 20051001 10
We generated a comprehensive picture of protease substrates in anti-Fas-treated apoptotic human Jurkat T lymphocytes. We used combined fractional diagonal chromatography (COFRADIC) sorting of protein amino-terminal peptides coupled to oxygen-16 or oxygen-18 differential labeling. We identified protease substrates and located the exact cleavage sites within processed proteins. Our analysis yielded 1,834 protein identifications and located 93 cleavage sites in 71 proteins. Indirect evidence of apo ...[more]
