Characterization of the Mouse Brain Proteome Using Global Proteomic Analysis Complemented with Cysteinyl-Peptide Enrichment
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ ion trap mass spectrometer
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: 
DAVE THORNEYCROFT
LAB HEAD: DAVE THORNEYCROFT
PROVIDER: PRD000010 | Pride | 2006-01-24
REPOSITORIES: Pride
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Characterization of the mouse brain proteome using global proteomic analysis complemented with cysteinyl-peptide enrichment.
Wang Haixing H Qian Wei-Jun WJ Chin Mark H MH Petyuk Vladislav A VA Barry Richard C RC Liu Tao T Gritsenko Marina A MA Mottaz Heather M HM Moore Ronald J RJ Camp Ii David G DG Khan Arshad H AH Smith Desmond J DJ Smith Richard D RD
Journal of proteome research 20060201 2
We report a global proteomic approach for analyzing brain tissue and for the first time a comprehensive characterization of the whole mouse brain proteome. Preparation of the whole brain sample incorporated a highly efficient cysteinyl-peptide enrichment (CPE) technique to complement a global enzymatic digestion method. Both the global and the cysteinyl-enriched peptide samples were analyzed by SCX fractionation coupled with reversed phase LC-MS/MS analysis. A total of 48,328 different peptides ...[more]
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