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Trypanosome flagellar proteome

ABSTRACT: This study investigates the proteome of the flagellum of the parasite Trypanosoma brucei. This parasite is the causative agent in African trypanosomiasis. This structure contains a 9 + 2 axoneme structure, highly conserved throughout eukaryotic evolution. The kinetoplastid flagellum additionally has a trypanasome-specific structure, the paraflagellar rod. Here, we have performed analyses based upon 1D- and 2D-gel separation followed by LC-MS/MS identification of peptides associated with flagellar proteins.

INSTRUMENT(S): instrument model, Waters instrument model

ORGANISM(S): Trypanosoma Brucei

DISEASE(S): Trypanosomiasis

SUBMITTER: DAVE THORNEYCROFT

PROVIDER: PRD000017 | Pride | 2006-07-04

REPOSITORIES: Pride

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Flagellar motility is required for the viability of the bloodstream trypanosome.

Broadhead Richard R Dawe Helen R HR Farr Helen H Griffiths Samantha S Hart Sarah R SR Portman Neil N Shaw Michael K MK Ginger Michael L ML Gaskell Simon J SJ McKean Paul G PG Gull Keith K

Nature 20060301 7081

The 9 + 2 microtubule axoneme of flagella and cilia represents one of the most iconic structures built by eukaryotic cells and organisms. Both unity and diversity are present among cilia and flagella on the evolutionary as well as the developmental scale. Some cilia are motile, whereas others function as sensory organelles and can variously possess 9 + 2 and 9 + 0 axonemes and other associated structures. How such unity and diversity are reflected in molecular repertoires is unclear. The flagell ...[more]

PMID: 16525475

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