Dataset Information
Proteomes of Bovine Sperm from Bulls of Different Fertility
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model, LCQ Deca XP Plus
ORGANISM(S): Bos Taurus (bovine)
SUBMITTER: 
DAVE THORNEYCROFT
PROVIDER: PRD000024 | Pride | 2011-04-15
REPOSITORIES: Pride
Publications
Differential detergent fractionation for non-electrophoretic eukaryote cell proteomics.
Journal of proteome research 20050301 2
Differential detergent fractionation (DDF), which relies on detergents to sequentially extract proteins from eukaryotic cells, has been used to increase proteome coverage of 2D-PAGE. Here, we used DDF extraction in conjunction with the nonelectrophoretic proteomics method of liquid chromatography and electrospray ionization tandem mass spectrometry. We demonstrate that DDF can be used with 2D-LC ESI MS2 for comprehensive cellular proteomics, including a large proportion of membrane proteins. Com ...[more]
