Proteomics

Dataset Information

0

Sequential Labeling for Protein Quantitation, Raijmakers et al.


ABSTRACT: Not available

INSTRUMENT(S): LTQ FT, LTQ Orbitrap, instrument model

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Reinout Raijmakers  

PROVIDER: PRD000045 | Pride | 2008-02-29

REPOSITORIES: Pride

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Publications

Automated online sequential isotope labeling for protein quantitation applied to proteasome tissue-specific diversity.

Raijmakers Reinout R   Berkers Celia R CR   de Jong Annemieke A   Ovaa Huib H   Heck Albert J R AJ   Mohammed Shabaz S  

Molecular & cellular proteomics : MCP 20080604 9


Quantitation of protein abundance is a vital component in the proteomic analysis of biological systems, which can be achieved by differential stable isotopic labeling. To analyze tissue-derived samples, the isotopic labeling can be performed using chemical labeling of the peptides post-digestion. Standard chemical labeling procedures often require many manual sample handling steps, reducing the accuracy of measurements. Here, we describe a fully automated, online (in nanoLC columns), labeling pr  ...[more]

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