Dataset Information
Straightforward peptide sequencing by MALDI-MS/MS using a Lys-N metallopeptidase - Boersema et al. 2008
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: 
Paul Boersema
PROVIDER: PRD000052 | Pride | 2008-12-02
REPOSITORIES: Pride
Publications
Straightforward and de novo peptide sequencing by MALDI-MS/MS using a Lys-N metalloendopeptidase.
Molecular & cellular proteomics : MCP 20081129 4
In this work, we explore the potential of the metalloendopeptidase Lys-N for MALDI-MS/MS proteomics applications. Initially we digested a HEK293 cellular lysate with Lys-N and, for comparison, in parallel with the protease Lys-C. The resulting peptides were separated by strong cation exchange to enrich and isolate peptides containing a single N-terminal lysine. MALDI-MS/MS analysis of these peptides yielded CID spectra with clear and often complete sequence ladders of b-ions. To test the applica ...[more]
