Dataset Information
Projects of the Clinical Proteomics Laboratories at the Medical University of Vienna (CPL/MUW)
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model, Agilent instrument model
ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human) Mus Musculus (mouse)
TISSUE(S): Kupffer Cell, Mononuclear Cell, Blood Plasma, Monocyte, Leukocyte, Hepg2 Cell, Liver, Melanocyte, Blood, Melanoma Cell Line, Erythrocyte, Platelet, Primary Cell, Hep-3b Cell, Hepatocyte, T Cell, Dendritic Cell, Keratinocyte, Skin, Neutrophil, Fibroblast, Bone Marrow, Blood Vessel Endothelial Cell
SUBMITTER: 
Christopher Gerner
PROVIDER: PRD000053 | Pride | 2012-05-02
REPOSITORIES: Pride
Publications
Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B.
Journal of proteome research 20100101 1
Hepatocytes are known to express a large number of characteristic proteins. Transformed and cultured hepatocytes only partially maintain functional cell differentiation characteristics, which can be assessed by proteome profiling. Here, we applied 2D-PAGE analysis in addition to shotgun proteomics to assess the functional cell state of primary human hepatocytes (PHH), HepG2 and Hep3B cells. Out of a total of 1995 proteins identified in the cytoplasm of these cells, we filtered 107 proteins which ...[more]
