Dataset Information
Triplex stable isotope dimethyl labelling - Boersema et al.
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ FT, LTQ Orbitrap, instrument model
ORGANISM(S): Rattus Norvegicus (rat) Mus Musculus (mouse)
TISSUE(S): Erythroid Lineage Cell, Skeletal Muscle
SUBMITTER: 
Phil Jones
PROVIDER: PRD000055 | Pride | 2008-09-23
REPOSITORIES: Pride
Publications
Triplex protein quantification based on stable isotope labeling by peptide dimethylation applied to cell and tissue lysates.
Proteomics 20081101 22
Stable isotope labeling is at present one of the most powerful methods in quantitative proteomics. Stable isotope labeling has been performed at both the protein as well as the peptide level using either metabolic or chemical labeling. Here, we present a straightforward and cost-effective triplex quantification method that is based on stable isotope dimethyl labeling at the peptide level. Herein, all proteolytic peptides are chemically labeled at their alpha- and epsilon-amino groups. We use thr ...[more]
