Understanding bovine germinal vesicle (GV) oocyte and cumulus cell communication... A systems biology approach

ABSTRACT: Triplicate samples of oocyte and cumulus cells were isolated from follicles of 2-8 mm, containing oocytes with several layers of cumulus cells were collected from ovaries. Proteins were extracted using DDF and digested using trypsin. The peptides were desalted, separated using in-line SCX-RPLC and analyzed using ion trap MS/MS. Proteins were identified using TurboSEQUEST 3.2

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cumulus Cell

SUBMITTER: Divyaswetha Peddinti  

LAB HEAD: Divyaswetha Peddinti

PROVIDER: PRD000082 | Pride | 2011-04-15

REPOSITORIES: Pride