Proteomics

Dataset Information

0

Phosphochip Q-ToF analysis of primary human leukocytes, Raijmakers et al.


ABSTRACT: Not available

INSTRUMENT(S): instrument model, Agilent instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte, Osteosarcoma Cell Line, Blood

SUBMITTER: Reinout Raijmakers  

PROVIDER: PRD000118 | Pride | 2009-12-01

REPOSITORIES: Pride

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Publications

Exploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometer.

Raijmakers Reinout R   Kraiczek Karsten K   de Jong Ad P AP   Mohammed Shabaz S   Heck Albert J R AJ  

Analytical chemistry 20100201 3


The study of protein phosphorylation events is one of the most important challenges in proteome analysis. Despite the importance of phosphorylation for many regulatory processes in cells and many years of phosphoprotein and phosphopeptide research, the identification and characterization of phosphorylation by mass spectrometry is still a challenging task. Recently, we introduced an approach that facilitates the analysis of phosphopeptides by performing automated, online, TiO(2) enrichment of pho  ...[more]

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