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Bradyrhizobium japonicum proteomic reference map, and Vigna mungo leaf proteome map

ABSTRACT: DISCLAIMER: This project actually contains two separate and independent assays by mistake. They should be not be considered together.

Assay 9769 - Bradyrhizobium japonicum proteomic reference map PMID : 20806226
Assay 15318 - Vigna mungo leaf proteome map PMID : 23587433

INSTRUMENT(S): Maldi-Tof-Tof, Bruker Autoflex II TOF/TOF

ORGANISM(S): Vigna Mungo (rice Bean) (black Gram)

SUBMITTER: J.S.S. Batista

LAB HEAD: J.S.S. Batista

PROVIDER: PRD000119 | Pride | 2011-11-15

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_15318.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_15318.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_9769.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_9769.xml.gz	Xml

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Publications

Towards a two-dimensional proteomic reference map of Bradyrhizobium japonicum CPAC 15: spotlighting "hypothetical proteins".

Batista Jesiane Stefânia da Silva JS Torres Adalgisa Ribeiro AR Hungria Mariangela M

Proteomics 20100901 17

The economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) - used in commercial inoculants for application to soybean crops in Brazil - grown under in vitro conditions. CPAC 15 belongs to the same serogro ...[more]

PMID: 20806226

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Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum

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Characterization of the Bradyrhizobium japonicum NtrC regulon by DNA Microarray analysis.

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Characterization of the Bradyrhizobium japonicum NtrC regulon by DNA Microarray analysis.

Project description:The Bradyrhizobium japonicum NtrC regulatory protein influences gene expression in response to changes in intracellular nitrogen status. Under conditions of low nitrogen, phosphorylation of NtrC results in up-regulation of a number of genes involved in nitrogen metabolism and nitrogen acquisition. To better define the exact nature of NtrC’s influence on gene expression, a ntrC mutation was created in B. japonicum and transcriptional profiling was performed by DNA microarray analysis of both the mutant and wild type strains. Bradyrhizobium japonicum USDA 110 and a ntrC mutant in the USDA 110 background were cultured in minimal medium supplemented with either 10mM glutamate (low nitrogen) or 10mM ammonium and 10mM glutamate (high nitrogen) as nitrogen sources. Four comparisons were performed: wild type high nitrogen vs. mutant high nitrogen, wild type low nitrogen vs. wild type high nitrogen, wild type low nitrogen vs. mutant low nitrogen, and mutant low nitrogen vs. mutant high nitrogen. For each of the four comparisons, three biological replicates were prepared for each strain and dye swap replications were performed for each hybridization producing a total of six arrays per comparison and 24 arrays in total.

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Project description:Elevated atmospheric CO2 can influence the structure and function of rhizosphere microorganisms by altering root growth and the quality and quantity of compounds released into the rhizosphere via root exudation. In these studies we investigated the transcriptional responses of Bradyrhizobium japonicum cells growing in the rhizosphere of soybean plants exposed to elevated atmospheric CO2. Transciptomic expression profiles indicated that genes involved in carbon/nitrogen metabolism, and FixK2-associated genes, including those involved in nitrogen fixation, microanaerobic respiration, respiratory nitrite reductase, and heme biosynthesis, were significantly up-regulated under conditions of elevated CO2, relative to plants and bacteria grown under ambient CO2 growth conditions. The expression profile of genes involved in lipochitinoligosaccharide Nod factor biosynthesis and negative transcriptional regulators of nodulation genes, nolA and nodD2, were also influenced by plant growth under conditions of elevated CO2. Taken together, results of these studies indicate that growth of soybeans under conditions of elevated atmospheric CO2 influences gene expressions in B. japonicum in the soybean rhizosphere, resulting in changes to carbon/nitrogen metabolism, respiration, and nodulation efficiency. Bradyrhizobium japonicum strains were grown in the soybean rhizosphere under two different CO2 concentrations. Transcriptional profiling of B. japonicum was compared between cells grown under elevated CO2 and ambient conditions. Four biological replicates of each treatment were prepared, and four microarray slides were used for each strain.

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