Dataset Information
C. elegans genome annotation project
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model, LTQ
ORGANISM(S): Caenorhabditis Elegans
SUBMITTER: 
Gennifer Merrihew
PROVIDER: PRD000122 | Pride | 2009-12-01
REPOSITORIES: Pride
Publications
Use of shotgun proteomics for the identification, confirmation, and correction of C. elegans gene annotations.
Genome research 20080724 10
We describe a general mass spectrometry-based approach for gene annotation of any organism and demonstrate its effectiveness using the nematode Caenorhabditis elegans. We detected 6779 C. elegans proteins (67,047 peptides), including 384 that, although annotated in WormBase WS150, lacked cDNA or other prior experimental support. We also identified 429 new coding sequences that were unannotated in WS150. Nearly half (192/429) of the new coding sequences were confirmed with RT-PCR data. Thirty-thr ...[more]
